Gene description for Abcf1
Gene name ATP-binding cassette, sub-family F (GCN20), member 1
Gene symbol Abcf1
Other names/aliases AU041969
Abc50
D17Wsu166e
GCN20
Species Mus musculus
 Database cross references - Abcf1
ExoCarta ExoCarta_224742
Vesiclepedia VP_224742
Entrez Gene 224742
UniProt Q6P542  
 Abcf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Abcf1
Molecular Function
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    translation activator activity GO:0008494 ISO
    translation activator activity GO:0008494 ISS
    ATP hydrolysis activity GO:0016887 IEA
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
Biological Process
    translational initiation GO:0006413 ISO
    translational initiation GO:0006413 ISS
    ribosome biogenesis GO:0042254 ISS
    positive regulation of translation GO:0045727 ISO
    positive regulation of translation GO:0045727 ISS
Subcellular Localization
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 ISO
 Experiment description of studies that identified Abcf1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Abcf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 JADE1  
Affinity Capture-MS Homo sapiens
3 Tmem173  
Proximity Label-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Edc4  
Co-fractionation Mus musculus
6 Hnrnpm 76936
Co-fractionation Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Abcf1 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Transport of small molecules IEA Reactome





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