Gene description for RAP1GAP2
Gene name RAP1 GTPase activating protein 2
Gene symbol RAP1GAP2
Other names/aliases GARNL4
RAP1GA3
Species Homo sapiens
 Database cross references - RAP1GAP2
ExoCarta ExoCarta_23108
Vesiclepedia VP_23108
Entrez Gene 23108
HGNC 29176
UniProt Q684P5  
 RAP1GAP2 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for RAP1GAP2
Molecular Function
    GTPase activator activity GO:0005096 EXP
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
Biological Process
    adaptive immune response GO:0002250 TAS
    regulation of cell size GO:0008361 IGI
    regulation of cell size GO:0008361 IMP
    regulation of small GTPase mediated signal transduction GO:0051056 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IGI
    nuclear membrane GO:0031965 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified RAP1GAP2 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAP1GAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XPO1 7514
Affinity Capture-MS Homo sapiens
2 RAP1GAP 5909
Affinity Capture-MS Homo sapiens
3 MEOX2  
Two-hybrid Homo sapiens
4 YWHAE 7531
Two-hybrid Homo sapiens
5 YWHAZ 7534
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which RAP1GAP2 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Immune System TAS Reactome
Rap1 signalling TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here