Gene description for GGA3
Gene name golgi-associated, gamma adaptin ear containing, ARF binding protein 3
Gene symbol GGA3
Other names/aliases -
Species Homo sapiens
 Database cross references - GGA3
ExoCarta ExoCarta_23163
Vesiclepedia VP_23163
Entrez Gene 23163
HGNC 17079
MIM 606006
UniProt Q9NZ52  
 GGA3 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for GGA3
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IDA
    phosphatidylinositol binding GO:0035091 IEA
    ubiquitin binding GO:0043130 IDA
    protein-containing complex binding GO:0044877 IDA
    protein transporter activity GO:0140318 IDA
Biological Process
    protein targeting to lysosome GO:0006622 IPI
    intracellular protein transport GO:0006886 NAS
    Golgi to plasma membrane transport GO:0006893 IBA
    protein catabolic process GO:0030163 IEA
    regulation of protein stability GO:0031647 IDA
    protein destabilization GO:0031648 IDA
    protein localization to cell surface GO:0034394 IBA
    protein localization to cell surface GO:0034394 IMP
    Golgi to plasma membrane protein transport GO:0043001 IMP
    negative regulation of amyloid-beta formation GO:1902430 IDA
    positive regulation of lysosomal protein catabolic process GO:1905167 TAS
Subcellular Localization
    lysosome GO:0005764 IDA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IBA
    trans-Golgi network GO:0005802 IDA
    endosome membrane GO:0010008 TAS
    early endosome membrane GO:0031901 TAS
    protein-containing complex GO:0032991 IDA
    recycling endosome membrane GO:0055038 TAS
 Experiment description of studies that identified GGA3 in sEVs
1
Experiment ID 224
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for GGA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SYNRG 11276
Protein-peptide Homo sapiens
2 LITAF 9516
Two-hybrid Homo sapiens
3 STX6 10228
Proximity Label-MS Homo sapiens
4 RNF43  
Proximity Label-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 ZNF497  
Two-hybrid Homo sapiens
7 AP2B1 163
Proximity Label-MS Homo sapiens
8 STAB1  
Reconstituted Complex Homo sapiens
9 TBC1D3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 EBAG9 9166
Proximity Label-MS Homo sapiens
11 NEDD4 4734
Affinity Capture-Western Homo sapiens
12 TMEM59 9528
Affinity Capture-MS Homo sapiens
13 HAUS4  
Co-fractionation Homo sapiens
14 PTGDS  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
15 RNF11 26994
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Co-localization Homo sapiens
16 PLEKHB2 55041
Two-hybrid Homo sapiens
17 RAB5A 5868
Proximity Label-MS Homo sapiens
18 GJA1 2697
Proximity Label-MS Homo sapiens
19 BACE1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 PFN1 5216
Proximity Label-MS Homo sapiens
21 RAB11A 8766
Proximity Label-MS Homo sapiens
22 CRK 1398
Affinity Capture-Western Homo sapiens
23 TOLLIP 54472
Reconstituted Complex Homo sapiens
24 M6PR 4074
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
25 ATG12  
Proximity Label-MS Homo sapiens
26 CDH1 999
Proximity Label-MS Homo sapiens
27 TSG101 7251
Two-hybrid Homo sapiens
28 ARF3 377
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 SQSTM1 8878
Proximity Label-MS Homo sapiens
30 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
31 ITCH 83737
Affinity Capture-Western Homo sapiens
32 ARF1 375
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
33 Arf6 11845
FRET Mus musculus
34 CLTB 1212
Proximity Label-MS Homo sapiens
35 DDX58 23586
Affinity Capture-RNA Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 RAB4A 5867
Proximity Label-MS Homo sapiens
38 DAZAP2  
Two-hybrid Homo sapiens
39 LAPTM5 7805
Affinity Capture-Western Homo sapiens
40 LAMTOR1 55004
Proximity Label-MS Homo sapiens
41 ARF6 382
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 B3GAT1  
Proximity Label-MS Homo sapiens
43 MOV10 4343
Affinity Capture-RNA Homo sapiens
44 MYO6 4646
Proximity Label-MS Homo sapiens
45 TRIM23 373
Two-hybrid Homo sapiens
46 MON2 23041
Affinity Capture-Western Homo sapiens
47 SORT1 6272
Affinity Capture-Western Homo sapiens
48 CLTA 1211
Proximity Label-MS Homo sapiens
49 PTGDR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 IGF2R 3482
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
51 PACS1 55690
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 MET 4233
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
53 IMPDH2 3615
Proximity Label-MS Homo sapiens
54 BICD1 636
Proximity Label-MS Homo sapiens
55 GGA2 23062
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
56 KRAS 3845
Synthetic Lethality Homo sapiens
57 ACBD5 91452
Proximity Label-MS Homo sapiens
58 CLTC 1213
Reconstituted Complex Homo sapiens
59 P4HA3 283208
Two-hybrid Homo sapiens
60 ATG9A 79065
Proximity Label-MS Homo sapiens
61 RABEP1 9135
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
62 UBC 444874
Reconstituted Complex Bos taurus
63 CLINT1 9685
Protein-peptide Homo sapiens
64 GGA1 26088
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
65 VPS18 57617
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
66 RNF214  
Two-hybrid Homo sapiens
67 CDKN2A 1029
FRET Homo sapiens
68 VPS11 55823
Biochemical Activity Homo sapiens
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