Gene description for Atp1a3
Gene name ATPase, Na+/K+ transporting, alpha 3 polypeptide
Gene symbol Atp1a3
Other names/aliases Atpa-2
Species Mus musculus
 Database cross references - Atp1a3
ExoCarta ExoCarta_232975
Vesiclepedia VP_232975
Entrez Gene 232975
UniProt Q6PIC6  
 Atp1a3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Microglia 16081791    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp1a3
Molecular Function
    amyloid-beta binding GO:0001540 ISO
    P-type sodium:potassium-exchanging transporter activity GO:0005391 IBA
    P-type sodium:potassium-exchanging transporter activity GO:0005391 IDA
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISO
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISS
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    D1 dopamine receptor binding GO:0031748 ISO
    heparan sulfate proteoglycan binding GO:0043395 IPI
    metal ion binding GO:0046872 IEA
    protein-folding chaperone binding GO:0051087 ISO
    P-type sodium:potassium-exchanging transporter activity involved in regulation of cardiac muscle cell membrane potential GO:0086037 IGI
Biological Process
    response to acid chemical GO:0001101 IMP
    potassium ion transport GO:0006813 ISO
    sodium ion transport GO:0006814 ISO
    intracellular sodium ion homeostasis GO:0006883 IBA
    intracellular sodium ion homeostasis GO:0006883 IDA
    intracellular sodium ion homeostasis GO:0006883 ISO
    response to stress GO:0006950 IMP
    gamma-aminobutyric acid signaling pathway GO:0007214 IMP
    behavior GO:0007610 IGI
    behavior GO:0007610 IMP
    learning GO:0007612 IMP
    memory GO:0007613 IMP
    circadian rhythm GO:0007623 IGI
    locomotory behavior GO:0007626 IMP
    blood circulation GO:0008015 IGI
    metabolic process GO:0008152 IMP
    determination of adult lifespan GO:0008340 IMP
    adult locomotory behavior GO:0008344 IMP
    visual learning GO:0008542 IDA
    visual learning GO:0008542 IMP
    response to temperature stimulus GO:0009266 IMP
    response to xenobiotic stimulus GO:0009410 IMP
    response to hormone GO:0009725 IMP
    response to lithium ion GO:0010226 IMP
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IEA
    response to activity GO:0014823 IMP
    transmission of nerve impulse GO:0019226 IMP
    calcium-mediated signaling GO:0019722 IMP
    L-ascorbic acid metabolic process GO:0019852 IGI
    L-ascorbic acid metabolic process GO:0019852 IMP
    thalamus development GO:0021794 IMP
    cerebral cortex development GO:0021987 IMP
    neurogenesis GO:0022008 IDA
    neurogenesis GO:0022008 IMP
    intracellular potassium ion homeostasis GO:0030007 IBA
    intracellular potassium ion homeostasis GO:0030007 ISO
    sleep GO:0030431 IMP
    brain-derived neurotrophic factor receptor signaling pathway GO:0031547 IMP
    social behavior GO:0035176 IMP
    ionotropic glutamate receptor signaling pathway GO:0035235 IMP
    multicellular organism growth GO:0035264 IDA
    multicellular organism growth GO:0035264 IMP
    response to potassium ion GO:0035864 IMP
    response to immobilization stress GO:0035902 IMP
    sodium ion export across plasma membrane GO:0036376 IBA
    sodium ion export across plasma membrane GO:0036376 IDA
    sodium ion export across plasma membrane GO:0036376 ISO
    locomotion GO:0040011 IMP
    response to chemical GO:0042221 IMP
    fear response GO:0042596 IDA
    fear response GO:0042596 IMP
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IMP
    pore complex assembly GO:0046931 IMP
    carboxylic acid transport GO:0046942 IMP
    habituation GO:0046959 IMP
    regulation of short-term neuronal synaptic plasticity GO:0048172 IMP
    neuromuscular process controlling balance GO:0050885 IDA
    neuromuscular process controlling balance GO:0050885 IMP
    cognition GO:0050890 IMP
    neuromuscular process GO:0050905 IMP
    response to electrical stimulus GO:0051602 IMP
    membrane depolarization GO:0051899 IMP
    cardiac muscle contraction GO:0060048 IDA
    motor behavior GO:0061744 IMP
    ERK1 and ERK2 cascade GO:0070371 IMP
    potassium ion transmembrane transport GO:0071805 IEA
    response to monoamine GO:0071867 IMP
    regulation of cardiac muscle cell membrane potential GO:0086036 IGI
    response to environmental enrichment GO:0090648 IMP
    walking behavior GO:0090659 IMP
    proton transmembrane transport GO:1902600 IBA
    response to methamphetamine hydrochloride GO:1904313 IMP
    cellular response to amyloid-beta GO:1904646 ISO
    neuron projection maintenance GO:1990535 ISO
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 ISO
Subcellular Localization
    photoreceptor inner segment GO:0001917 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISS
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    sodium:potassium-exchanging ATPase complex GO:0005890 IDA
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    cell surface GO:0009986 IDA
    membrane GO:0016020 ISO
    axon GO:0030424 ISO
    organelle membrane GO:0031090 ISO
    protein-containing complex GO:0032991 IDA
    sarcolemma GO:0042383 IDA
    presynaptic membrane GO:0042734 ISO
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
    myelin sheath GO:0043209 ISO
    calyx of Held GO:0044305 ISO
    dendritic spine neck GO:0044326 ISO
    dendritic spine head GO:0044327 ISO
    synapse GO:0045202 ISO
    synapse GO:0045202 ISS
    postsynapse GO:0098794 ISO
    neuron to neuron synapse GO:0098984 IDA
 Experiment description of studies that identified Atp1a3 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 14
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 33
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp1a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vamp2  
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 MAPT  
Reconstituted Complex Homo sapiens
6 Prnp 19122
Affinity Capture-MS Mus musculus
7 Aph1b  
Affinity Capture-MS Mus musculus
8 Aph1a 226548
Affinity Capture-MS Mus musculus
9 LDLRAD3  
Reconstituted Complex Homo sapiens
10 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
11 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here