Gene description for SZT2
Gene name seizure threshold 2 homolog (mouse)
Gene symbol SZT2
Other names/aliases C1orf84
EIEE18
KIAA0467
SZT2A
SZT2B
Species Homo sapiens
 Database cross references - SZT2
ExoCarta ExoCarta_23334
Vesiclepedia VP_23334
Entrez Gene 23334
HGNC 29040
MIM 615463
UniProt Q5T011  
 SZT2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for SZT2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    central nervous system development GO:0007417 ISS
    post-embryonic development GO:0009791 ISS
    corpus callosum morphogenesis GO:0021540 IMP
    cellular response to amino acid starvation GO:0034198 IMP
    cellular response to glucose starvation GO:0042149 IMP
    pigmentation GO:0043473 IEA
    protein localization to lysosome GO:0061462 IMP
    regulation of superoxide dismutase activity GO:1901668 ISS
    negative regulation of TORC1 signaling GO:1904262 IMP
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 ISS
    GATOR2 complex GO:0061700 IDA
    KICSTOR complex GO:0140007 IDA
    KICSTOR complex GO:0140007 NAS
    GATOR1 complex GO:1990130 IDA
 Experiment description of studies that identified SZT2 in sEVs
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 275
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SZT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 CD3D 915
Affinity Capture-MS Homo sapiens
3 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KPTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 FASN 2194
Negative Genetic Homo sapiens
6 NPRL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 RAD23A 5886
Affinity Capture-MS Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NPRL3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 ITFG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 RAB9A 9367
Proximity Label-MS Homo sapiens
14 REL 5966
Two-hybrid Homo sapiens
15 LAMP1 3916
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
16 TSGA10IP  
Affinity Capture-MS Homo sapiens
17 DEPDC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 LAMP2 3920
Proximity Label-MS Homo sapiens
Co-localization Homo sapiens
19 ENTHD2  
Two-hybrid Homo sapiens
20 SHMT2 6472
Affinity Capture-RNA Homo sapiens
21 DUSP16  
Affinity Capture-MS Homo sapiens
22 CRX  
Two-hybrid Homo sapiens
23 TCF4  
Two-hybrid Homo sapiens
24 C12orf66  
Affinity Capture-Western Homo sapiens
25 WDR24  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 RAB7A 7879
Co-localization Homo sapiens
27 Sesn2  
Affinity Capture-MS Mus musculus
28 WDR59 79726
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
29 ZNF655  
Two-hybrid Homo sapiens
30 ZUFSP 221302
Affinity Capture-MS Homo sapiens
31 MIOS 54468
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
32 SEH1L 81929
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 C7orf60 154743
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SESN2 83667
Affinity Capture-Western Homo sapiens
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