Gene description for SPIDR
Gene name scaffolding protein involved in DNA repair
Gene symbol SPIDR
Other names/aliases KIAA0146
Species Homo sapiens
 Database cross references - SPIDR
ExoCarta ExoCarta_23514
Vesiclepedia VP_23514
Entrez Gene 23514
HGNC 28971
MIM 615384
UniProt Q14159  
 SPIDR identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for SPIDR
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    double-strand break repair via homologous recombination GO:0000724 IBA
    double-strand break repair via homologous recombination GO:0000724 IMP
    DNA damage response GO:0006974 IDA
    regulation of double-strand break repair via homologous recombination GO:0010569 IDA
    positive regulation of protein-containing complex assembly GO:0031334 IDA
    regulation of establishment of protein localization to chromosome GO:0070202 IBA
    regulation of establishment of protein localization to chromosome GO:0070202 IDA
    cellular response to ionizing radiation GO:0071479 IDA
    cellular response to hydroxyurea GO:0072711 IDA
    cellular response to camptothecin GO:0072757 IDA
    positive regulation of double-strand break repair GO:2000781 IMP
Subcellular Localization
    nuclear chromosome GO:0000228 IBA
    nuclear chromosome GO:0000228 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified SPIDR in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for SPIDR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APEX1 328
Affinity Capture-RNA Homo sapiens
2 RNF38  
Affinity Capture-MS Homo sapiens
3 SWSAP1  
Affinity Capture-MS Homo sapiens
4 FAM83F 113828
Affinity Capture-MS Homo sapiens
5 FOXL1  
Affinity Capture-MS Homo sapiens
6 ZSWIM7  
Affinity Capture-MS Homo sapiens
7 DDX58 23586
Affinity Capture-RNA Homo sapiens
8 PLA2G10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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