Gene description for FTSJ1
Gene name FtsJ RNA methyltransferase homolog 1 (E. coli)
Gene symbol FTSJ1
Other names/aliases CDLIV
JM23
MRX44
MRX9
SPB1
TRMT7
Species Homo sapiens
 Database cross references - FTSJ1
ExoCarta ExoCarta_24140
Vesiclepedia VP_24140
Entrez Gene 24140
HGNC 13254
MIM 300499
UniProt Q9UET6  
 FTSJ1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FTSJ1
Molecular Function
    protein binding GO:0005515 IPI
    tRNA methyltransferase activity GO:0008175 IBA
    tRNA (guanine) methyltransferase activity GO:0016423 IDA
    tRNA 2'-O-methyltransferase activity GO:0106050 EXP
    tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase activity GO:0106340 EXP
    tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase activity GO:0106340 IMP
    S-adenosyl-L-methionine binding GO:1904047 IDA
Biological Process
    tRNA nucleoside ribose methylation GO:0002128 IMP
    wobble position ribose methylation GO:0002130 IDA
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 IMP
    tRNA modification GO:0006400 TAS
    neurogenesis GO:0022008 IDA
    tRNA methylation GO:0030488 IBA
    tRNA methylation GO:0030488 IGI
    tRNA methylation GO:0030488 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified FTSJ1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FTSJ1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 MECP2 4204
Affinity Capture-MS Homo sapiens
3 MRM1  
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 SIRT6  
Affinity Capture-MS Homo sapiens
6 LAMTOR1 55004
Proximity Label-MS Homo sapiens
7 USP43  
Affinity Capture-MS Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 HCST  
Affinity Capture-MS Homo sapiens
10 RPS19BP1  
Affinity Capture-MS Homo sapiens
11 CHRM3 1131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CDC37 11140
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CX3CL1  
Affinity Capture-MS Homo sapiens
14 CUL3 8452
Affinity Capture-MS Homo sapiens
15 DCAF13 25879
Affinity Capture-MS Homo sapiens
16 DMWD  
Affinity Capture-MS Homo sapiens
17 ARF6 382
Proximity Label-MS Homo sapiens
18 CXADR 1525
Proximity Label-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 WDR6 11180
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 APEX1 328
Affinity Capture-RNA Homo sapiens
22 PRKACA 5566
Proximity Label-MS Homo sapiens
23 DNAJC5 80331
Proximity Label-MS Homo sapiens
24 DNAJC5B  
Proximity Label-MS Homo sapiens
25 Cdc37 12539
Affinity Capture-MS Mus musculus
26 IBTK 25998
Affinity Capture-MS Homo sapiens
27 LAMP1 3916
Proximity Label-MS Homo sapiens
28 EPHA2 1969
Proximity Label-MS Homo sapiens
29 WDR5B  
Affinity Capture-MS Homo sapiens
30 ZFP91 80829
Affinity Capture-MS Homo sapiens
31 KRAS 3845
Proximity Label-MS Homo sapiens
32 PIK3R1 5295
Affinity Capture-MS Homo sapiens
33 RPL36AL 6166
Affinity Capture-MS Homo sapiens
34 DYSF 8291
Affinity Capture-MS Homo sapiens
35 GNL3L 54552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FTSJ1 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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