Gene description for Add2
Gene name adducin 2 (beta)
Gene symbol Add2
Other names/aliases beta-ADD
Species Rattus norvegicus
 Database cross references - Add2
ExoCarta ExoCarta_24171
Vesiclepedia VP_24171
Entrez Gene 24171
UniProt Q05764  
 Add2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Add2
Molecular Function
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    structural constituent of cytoskeleton GO:0005200 IEA
    structural constituent of cytoskeleton GO:0005200 ISO
    structural constituent of cytoskeleton GO:0005200 NAS
    calmodulin binding GO:0005516 IEA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    spectrin binding GO:0030507 IEA
    spectrin binding GO:0030507 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein dimerization activity GO:0046983 ISO
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
Biological Process
    monoatomic ion transport GO:0006811 IMP
    synapse assembly GO:0007416 IEA
    synapse assembly GO:0007416 ISO
    hemopoiesis GO:0030097 IEA
    hemopoiesis GO:0030097 ISO
    leukocyte migration GO:0050900 ISO
    leukocyte tethering or rolling GO:0050901 IEA
    leukocyte tethering or rolling GO:0050901 ISO
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IEA
    barbed-end actin filament capping GO:0051016 ISO
    actin filament bundle assembly GO:0051017 IEA
    actin filament bundle assembly GO:0051017 ISO
    protein-containing complex assembly GO:0065003 IEA
    protein-containing complex assembly GO:0065003 ISO
    protein-containing complex assembly GO:0065003 ISS
Subcellular Localization
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    F-actin capping protein complex GO:0008290 IEA
    F-actin capping protein complex GO:0008290 ISO
    postsynaptic density GO:0014069 IBA
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 ISO
    cytoplasmic vesicle GO:0031410 IEA
    cytoplasmic vesicle GO:0031410 ISO
    cytoplasmic vesicle GO:0031410 ISS
    plasma membrane raft GO:0044853 IEA
    plasma membrane raft GO:0044853 ISO
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Add2 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Add2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Add2 is involved
PathwayEvidenceSource
Miscellaneous transport and binding events IEA Reactome
Transport of small molecules IEA Reactome





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