Gene description for Gnb1
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 1
Gene symbol Gnb1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnb1
ExoCarta ExoCarta_24400
Vesiclepedia VP_24400
Entrez Gene 24400
UniProt P54311  
 Gnb1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gnb1
Molecular Function
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    signaling receptor complex adaptor activity GO:0030159 IBA
    spectrin binding GO:0030507 IDA
    protein-containing complex binding GO:0044877 ISO
    alkylglycerophosphoethanolamine phosphodiesterase activity GO:0047391 IDA
    GTPase binding GO:0051020 ISO
Biological Process
    signal transduction GO:0007165 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 IMP
    cell population proliferation GO:0008283 ISO
    cardiac muscle cell apoptotic process GO:0010659 IDA
    sensory perception of taste GO:0050909 ISO
    retina development in camera-type eye GO:0060041 ISO
    cellular response to hypoxia GO:0071456 IDA
Subcellular Localization
    photoreceptor outer segment GO:0001750 ISO
    photoreceptor inner segment GO:0001917 IDA
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IDA
    heterotrimeric G-protein complex GO:0005834 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 TAS
    dendrite GO:0030425 IDA
    photoreceptor outer segment membrane GO:0042622 IDA
    cell body GO:0044297 IDA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Gnb1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gnb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cacna1a  
FRET Rattus norvegicus
Two-hybrid Rattus norvegicus
2 NME2 4831
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
3 Snca  
Affinity Capture-MS Rattus norvegicus
4 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
5 Sumo3  
Affinity Capture-MS Rattus norvegicus
6 STXBP5L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Gnb1 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels IEA Reactome
Activation of GABAB receptors IEA Reactome
Activation of the phototransduction cascade IEA Reactome
ADP signalling through P2Y purinoceptor 12 IEA Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Aquaporin-mediated transport IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Class B/2 (Secretin family receptors) IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G protein gated Potassium channels IEA Reactome
G-protein activation IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GABA B receptor activation IEA Reactome
GABA receptor activation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors IEA Reactome
GPCR downstream signalling IEA Reactome
GPCR ligand binding IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits IEA Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels IEA Reactome
Metabolism IEA Reactome
Metabolism of proteins IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Potassium Channels IEA Reactome
Protein folding IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Sensory Perception IEA Reactome
Sensory perception of sweet, bitter, and umami (glutamate) taste IEA Reactome
Sensory perception of taste IEA Reactome
Signal amplification IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by WNT IEA Reactome
The phototransduction cascade IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome
Visual phototransduction IEA Reactome





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