Gene description for Kras
Gene name Kirsten rat sarcoma viral oncogene
Gene symbol Kras
Other names/aliases Kras2
c-Ki-ras
p21
Species Rattus norvegicus
 Database cross references - Kras
ExoCarta ExoCarta_24525
Vesiclepedia VP_24525
Entrez Gene 24525
UniProt P08644  
 Kras identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Kras
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GMP binding GO:0019002 IDA
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 IDA
    LRR domain binding GO:0030275 IDA
    identical protein binding GO:0042802 ISO
    protein-membrane adaptor activity GO:0043495 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    liver development GO:0001889 IEP
    positive regulation of protein phosphorylation GO:0001934 ISO
    signal transduction GO:0007165 IEA
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 ISO
    female pregnancy GO:0007565 IEP
    cell population proliferation GO:0008283 ISO
    visual learning GO:0008542 ISO
    response to gravity GO:0009629 IEP
    gene expression GO:0010467 ISO
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of gene expression GO:0010628 ISO
    glial cell proliferation GO:0014009 ISO
    Rac protein signal transduction GO:0016601 ISO
    cytokine-mediated signaling pathway GO:0019221 IMP
    forebrain astrocyte development GO:0021897 ISO
    actin cytoskeleton organization GO:0030036 ISO
    negative regulation of epithelial cell differentiation GO:0030857 ISO
    regulation of synaptic transmission, GABAergic GO:0032228 ISO
    positive regulation of Rac protein signal transduction GO:0035022 ISO
    response to isolation stress GO:0035900 IEP
    skeletal muscle cell differentiation GO:0035914 ISO
    positive regulation of MAP kinase activity GO:0043406 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 ISO
    homeostasis of number of cells within a tissue GO:0048873 ISO
    positive regulation of nitric-oxide synthase activity GO:0051000 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    striated muscle cell differentiation GO:0051146 ISO
    response to glucocorticoid GO:0051384 IEP
    response to mineralocorticoid GO:0051385 IEP
    neuron apoptotic process GO:0051402 ISO
    myoblast proliferation GO:0051450 IEP
    cardiac muscle cell proliferation GO:0060038 IEP
    positive regulation of glial cell proliferation GO:0060252 ISO
    epithelial tube branching involved in lung morphogenesis GO:0060441 ISO
    type I pneumocyte differentiation GO:0060509 ISO
    positive regulation of cellular senescence GO:2000774 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Kras in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Kras
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cdc20  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
2 Cdk2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
3 Cdc27  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Ccne1  
Affinity Capture-Western Rattus norvegicus
5 Ccnd2  
Affinity Capture-Western Rattus norvegicus
6 Mib1  
Affinity Capture-MS Rattus norvegicus
7 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
8 Ubc  
Affinity Capture-Western Rattus norvegicus
9 Skp2  
Affinity Capture-Western Rattus norvegicus
10 Cdk4  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Kras is involved
PathwayEvidenceSource
Activation of RAS in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
Ca2+ pathway IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
FLT3 Signaling IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MET activates RAS signaling IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Nervous system development IEA Reactome
p38MAPK events IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
RAS processing IEA Reactome
Regulation of RAS by GAPs IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB4 IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by MET IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF IEA Reactome
Signaling by WNT IEA Reactome
Signalling to ERKs IEA Reactome
Signalling to RAS IEA Reactome
Tie2 Signaling IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome





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