Gene description for Prpf19
Gene name pre-mRNA processing factor 19
Gene symbol Prpf19
Other names/aliases Prp19
Species Rattus norvegicus
 Database cross references - Prpf19
ExoCarta ExoCarta_246216
Vesiclepedia VP_246216
Entrez Gene 246216
UniProt Q9JMJ4  
 Prpf19 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Prpf19
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IBA
    protein binding GO:0005515 IPI
    ubiquitin-ubiquitin ligase activity GO:0034450 IEA
    ubiquitin-ubiquitin ligase activity GO:0034450 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
    ubiquitin protein ligase activity GO:0061630 ISS
Biological Process
    DNA damage checkpoint signaling GO:0000077 IEA
    DNA damage checkpoint signaling GO:0000077 ISO
    DNA damage checkpoint signaling GO:0000077 ISS
    protein polyubiquitination GO:0000209 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 IEA
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    spliceosomal complex assembly GO:0000245 IEA
    spliceosomal complex assembly GO:0000245 ISO
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IEA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    inner cell mass cell proliferation GO:0001833 IEA
    DNA repair GO:0006281 IEA
    double-strand break repair via nonhomologous end joining GO:0006303 IEA
    double-strand break repair via nonhomologous end joining GO:0006303 ISO
    double-strand break repair via nonhomologous end joining GO:0006303 ISS
    protein localization GO:0008104 IEA
    protein localization GO:0008104 ISO
    protein localization GO:0008104 ISS
    lipid biosynthetic process GO:0008610 IEA
    lipid biosynthetic process GO:0008610 ISO
    proteasomal protein catabolic process GO:0010498 IEA
    proteasomal protein catabolic process GO:0010498 ISO
    proteasomal protein catabolic process GO:0010498 ISS
    positive regulation of neuron differentiation GO:0045666 IDA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IEA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISO
    positive regulation of astrocyte differentiation GO:0048711 IMP
    protein K63-linked ubiquitination GO:0070534 IEA
    protein K63-linked ubiquitination GO:0070534 ISO
    protein K63-linked ubiquitination GO:0070534 ISS
Subcellular Localization
    Prp19 complex GO:0000974 IBA
    Prp19 complex GO:0000974 IEA
    Prp19 complex GO:0000974 ISO
    Prp19 complex GO:0000974 ISS
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    DNA replication factor A complex GO:0005662 IEA
    DNA replication factor A complex GO:0005662 ISO
    DNA replication factor A complex GO:0005662 ISS
    spliceosomal complex GO:0005681 IEA
    spliceosomal complex GO:0005681 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    lipid droplet GO:0005811 IEA
    spindle GO:0005819 IEA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    nuclear speck GO:0016607 ISS
    site of double-strand break GO:0035861 IEA
    site of double-strand break GO:0035861 ISO
    site of double-strand break GO:0035861 ISS
    U2-type catalytic step 1 spliceosome GO:0071006 IBA
    U2-type catalytic step 2 spliceosome GO:0071007 IEA
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Prpf19 in sEVs
1
Experiment ID 96
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prpf19
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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