Gene description for Vps4a
Gene name vacuolar protein sorting 4 homolog A (S. cerevisiae)
Gene symbol Vps4a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vps4a
ExoCarta ExoCarta_246772
Vesiclepedia VP_246772
Entrez Gene 246772
UniProt Q793F9  
 Vps4a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vps4a
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    small GTPase binding GO:0031267 IPI
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein targeting to lysosome GO:0006622 IEA
    protein targeting to lysosome GO:0006622 ISO
    vesicle budding from membrane GO:0006900 IEA
    vesicle budding from membrane GO:0006900 ISO
    nucleus organization GO:0006997 IEA
    nucleus organization GO:0006997 ISO
    endosome organization GO:0007032 ISO
    vacuole organization GO:0007033 IBA
    mitotic metaphase chromosome alignment GO:0007080 IEA
    mitotic metaphase chromosome alignment GO:0007080 ISO
    vesicle-mediated transport GO:0016192 ISO
    endosomal transport GO:0016197 IBA
    endosomal transport GO:0016197 IC
    endosomal transport GO:0016197 IEA
    endosomal transport GO:0016197 ISO
    intracellular cholesterol transport GO:0032367 IEA
    intracellular cholesterol transport GO:0032367 ISO
    negative regulation of cytokinesis GO:0032466 IEA
    negative regulation of cytokinesis GO:0032466 ISO
    negative regulation of cytokinesis GO:0032466 ISS
    regulation of protein localization GO:0032880 ISO
    endosomal vesicle fusion GO:0034058 IEA
    endosomal vesicle fusion GO:0034058 ISO
    multivesicular body assembly GO:0036258 IEA
    multivesicular body assembly GO:0036258 ISO
    viral budding via host ESCRT complex GO:0039702 IEA
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    mitotic cytokinesis checkpoint signaling GO:0044878 IEA
    mitotic cytokinesis checkpoint signaling GO:0044878 ISO
    mitotic cytokinesis checkpoint signaling GO:0044878 ISS
    viral budding from plasma membrane GO:0046761 IEA
    viral budding from plasma membrane GO:0046761 ISO
    cell division GO:0051301 ISO
    late endosomal microautophagy GO:0061738 IEA
    late endosomal microautophagy GO:0061738 ISO
    midbody abscission GO:0061952 IEA
    midbody abscission GO:0061952 ISO
    midbody abscission GO:0061952 ISS
    vesicle uncoating GO:0072319 IEA
    vesicle uncoating GO:0072319 ISO
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 IEA
    ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway GO:0090611 ISO
    chromatin looping GO:0140588 IEA
    regulation of protein localization to plasma membrane GO:1903076 IEA
    regulation of protein localization to plasma membrane GO:1903076 ISO
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 IEA
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
Subcellular Localization
    spindle pole GO:0000922 IEA
    spindle pole GO:0000922 ISO
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    endosome GO:0005768 ISO
    early endosome GO:0005769 IDA
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 ISO
    vacuolar membrane GO:0005774 IEA
    vacuolar membrane GO:0005774 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endosome membrane GO:0010008 ISO
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    late endosome membrane GO:0031902 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    Flemming body GO:0090543 IEA
    Flemming body GO:0090543 ISO
 Experiment description of studies that identified Vps4a in sEVs
1
Experiment ID 90
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vps4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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