Gene description for Tpm4
Gene name tropomyosin 4
Gene symbol Tpm4
Other names/aliases Tpm4.2
Tpm4.2cy
Species Rattus norvegicus
 Database cross references - Tpm4
ExoCarta ExoCarta_24852
Vesiclepedia VP_24852
Entrez Gene 24852
UniProt P09495  
 Tpm4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tpm4
Molecular Function
    actin binding GO:0003779 IEA
    identical protein binding GO:0042802 IDA
    protein homodimerization activity GO:0042803 IDA
    metal ion binding GO:0046872 IEA
    protein heterodimerization activity GO:0046982 IDA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    actin filament binding GO:0051015 IEA
    actin filament binding GO:0051015 ISO
Biological Process
    muscle contraction GO:0006936 IBA
    muscle contraction GO:0006936 TAS
    actin filament organization GO:0007015 IBA
    platelet formation GO:0030220 IEA
    platelet formation GO:0030220 ISO
    platelet formation GO:0030220 ISS
Subcellular Localization
    stress fiber GO:0001725 ISO
    podosome GO:0002102 IEA
    podosome GO:0002102 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    actin filament GO:0005884 IBA
    actin cytoskeleton GO:0015629 IDA
    cortical cytoskeleton GO:0030863 IEA
    cortical cytoskeleton GO:0030863 ISO
 Experiment description of studies that identified Tpm4 in sEVs
1
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tpm4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STXBP5L  
Affinity Capture-MS Homo sapiens
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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