Gene description for Gnas
Gene name GNAS complex locus
Gene symbol Gnas
Other names/aliases Gnas1
Gnpas
Nesp55
Species Rattus norvegicus
 Database cross references - Gnas
ExoCarta ExoCarta_24896
Vesiclepedia VP_24896
Entrez Gene 24896
UniProt P63095   Q792G6  
 Gnas identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Urine 20535238    
 Gene ontology annotations for Gnas
Molecular Function
    G-protein alpha-subunit binding GO:0001965 IPI
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    insulin-like growth factor receptor binding GO:0005159 IBA
    insulin-like growth factor receptor binding GO:0005159 IPI
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 TAS
    adenylate cyclase regulator activity GO:0010854 ISO
    adenylate cyclase activator activity GO:0010856 IDA
    adenylate cyclase activator activity GO:0010856 IEA
    adenylate cyclase activator activity GO:0010856 ISO
    adenylate cyclase activator activity GO:0010856 ISS
    protein domain specific binding GO:0019904 IPI
    G-protein beta-subunit binding GO:0031681 IDA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    beta-2 adrenergic receptor binding GO:0031698 IBA
    beta-2 adrenergic receptor binding GO:0031698 IPI
    D1 dopamine receptor binding GO:0031748 IBA
    D1 dopamine receptor binding GO:0031748 IPI
    D1 dopamine receptor binding GO:0031748 ISO
    mu-type opioid receptor binding GO:0031852 IBA
    mu-type opioid receptor binding GO:0031852 IDA
    mu-type opioid receptor binding GO:0031852 IPI
    ionotropic glutamate receptor binding GO:0035255 IBA
    ionotropic glutamate receptor binding GO:0035255 IPI
    alpha-tubulin binding GO:0043014 IDA
    metal ion binding GO:0046872 IEA
    alkylglycerophosphoethanolamine phosphodiesterase activity GO:0047391 IDA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IBA
    corticotropin-releasing hormone receptor 1 binding GO:0051430 IPI
Biological Process
    skeletal system development GO:0001501 ISO
    tissue homeostasis GO:0001894 IEA
    tissue homeostasis GO:0001894 ISO
    positive regulation of protein phosphorylation GO:0001934 IMP
    endochondral ossification GO:0001958 IEA
    endochondral ossification GO:0001958 ISO
    energy reserve metabolic process GO:0006112 IEA
    energy reserve metabolic process GO:0006112 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IBA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IDA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IEA
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 ISO
    adenylate cyclase-activating serotonin receptor signaling pathway GO:0007192 ISO
    sensory perception of chemical stimulus GO:0007606 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to xenobiotic stimulus GO:0009410 ISO
    post-embryonic development GO:0009791 ISO
    regulation of signal transduction GO:0009966 ISO
    positive regulation of sodium ion transport GO:0010765 IMP
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of insulin secretion GO:0032024 ISO
    response to prostaglandin E GO:0034695 ISO
    embryonic hindlimb morphogenesis GO:0035116 IEA
    embryonic hindlimb morphogenesis GO:0035116 ISO
    multicellular organism growth GO:0035264 IEA
    multicellular organism growth GO:0035264 ISO
    negative regulation of multicellular organism growth GO:0040015 ISO
    post-embryonic body morphogenesis GO:0040032 IEA
    post-embryonic body morphogenesis GO:0040032 ISO
    skin development GO:0043588 IEA
    skin development GO:0043588 ISO
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of osteoblast differentiation GO:0045669 ISO
    positive regulation of osteoclast differentiation GO:0045672 IEA
    positive regulation of osteoclast differentiation GO:0045672 ISO
    negative regulation of blood pressure GO:0045776 IMP
    developmental growth GO:0048589 ISO
    embryonic cranial skeleton morphogenesis GO:0048701 IEA
    embryonic cranial skeleton morphogenesis GO:0048701 ISO
    cognition GO:0050890 ISO
    cartilage development GO:0051216 IEA
    cartilage development GO:0051216 ISO
    bone development GO:0060348 ISO
    hair follicle placode formation GO:0060789 ISO
    platelet aggregation GO:0070527 ISO
    response to parathyroid hormone GO:0071107 IEA
    response to parathyroid hormone GO:0071107 ISO
    cellular response to glucagon stimulus GO:0071377 IEA
    cellular response to glucagon stimulus GO:0071377 ISO
    cellular response to prostaglandin E stimulus GO:0071380 IDA
    genomic imprinting GO:0071514 IEA
    genomic imprinting GO:0071514 ISO
    cellular response to catecholamine stimulus GO:0071870 IDA
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 ISO
    adenylate cyclase-activating adrenergic receptor signaling pathway GO:0071880 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISO
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    regulation of parathyroid hormone secretion GO:2000828 IEA
    regulation of parathyroid hormone secretion GO:2000828 ISO
Subcellular Localization
    ruffle GO:0001726 IDA
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IDA
    heterotrimeric G-protein complex GO:0005834 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IEA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IEA
    COPI-coated Golgi to ER transport vesicle GO:0030142 IDA
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    trans-Golgi network membrane GO:0032588 ISO
    sarcolemma GO:0042383 IDA
    neuronal cell body GO:0043025 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified Gnas in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnas
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gnas is involved
No pathways found





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