Gene description for Gnai1
Gene name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Gene symbol Gnai1
Other names/aliases BPGTPB
Species Rattus norvegicus
 Database cross references - Gnai1
ExoCarta ExoCarta_25686
Vesiclepedia VP_25686
Entrez Gene 25686
UniProt P10824  
 Gnai1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Gnai1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    magnesium ion binding GO:0000287 IMP
    G protein-coupled receptor binding GO:0001664 IDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 IMP
    GTP binding GO:0005525 ISO
    adenylate cyclase regulator activity GO:0010854 IEA
    adenylate cyclase regulator activity GO:0010854 ISO
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 IMP
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    D2 dopamine receptor binding GO:0031749 IEA
    D2 dopamine receptor binding GO:0031749 ISO
    G protein-coupled serotonin receptor binding GO:0031821 IBA
    G protein-coupled serotonin receptor binding GO:0031821 IPI
    GTPase activating protein binding GO:0032794 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IMP
    adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway GO:0007193 ISO
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 IEA
    adenylate cyclase-inhibiting serotonin receptor signaling pathway GO:0007198 ISO
    response to prostaglandin E GO:0034695 IEA
    response to prostaglandin E GO:0034695 ISO
    positive regulation of cholesterol biosynthetic process GO:0045542 IEA
    positive regulation of cholesterol biosynthetic process GO:0045542 ISO
    negative regulation of synaptic transmission GO:0050805 IMP
    cell division GO:0051301 IEA
    cell division GO:0051301 ISO
    cell division GO:0051301 ISS
    regulation of mitotic spindle organization GO:0060236 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    T cell migration GO:0072678 IEA
    T cell migration GO:0072678 ISO
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IDA
    neurotransmitter receptor localization to postsynaptic specialization membrane GO:0099645 IEP
    cellular response to forskolin GO:1904322 IMP
    positive regulation of protein localization to cell cortex GO:1904778 IEA
    positive regulation of protein localization to cell cortex GO:1904778 ISO
    positive regulation of protein localization to cell cortex GO:1904778 ISS
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IDA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IEA
    heterotrimeric G-protein complex GO:0005834 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    protein-containing complex GO:0032991 IDA
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 NAS
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 NAS
 Experiment description of studies that identified Gnai1 in sEVs
1
Experiment ID 225
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Gnai1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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