Gene description for Got2
Gene name glutamic-oxaloacetic transaminase 2, mitochondrial
Gene symbol Got2
Other names/aliases ASPATA
Species Rattus norvegicus
 Database cross references - Got2
ExoCarta ExoCarta_25721
Vesiclepedia VP_25721
Entrez Gene 25721
UniProt P00507  
 Got2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Got2
Molecular Function
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IBA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IDA
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 IMP
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISO
    L-aspartate:2-oxoglutarate aminotransferase activity GO:0004069 ISS
    phospholipid binding GO:0005543 IDA
    transaminase activity GO:0008483 IEA
    kynurenine-oxoglutarate transaminase activity GO:0016212 IEA
    amino acid binding GO:0016597 IDA
    enzyme binding GO:0019899 IDA
    pyridoxal phosphate binding GO:0030170 IDA
    pyridoxal phosphate binding GO:0030170 IEA
    pyridoxal phosphate binding GO:0030170 IMP
    carboxylic acid binding GO:0031406 IDA
    identical protein binding GO:0042802 IMP
Biological Process
    2-oxoglutarate metabolic process GO:0006103 ISS
    oxaloacetate metabolic process GO:0006107 IDA
    oxaloacetate metabolic process GO:0006107 ISO
    amino acid metabolic process GO:0006520 IDA
    amino acid metabolic process GO:0006520 IEA
    amino acid metabolic process GO:0006520 TAS
    aspartate metabolic process GO:0006531 IMP
    aspartate metabolic process GO:0006531 ISS
    aspartate biosynthetic process GO:0006532 ISO
    aspartate catabolic process GO:0006533 IBA
    aspartate catabolic process GO:0006533 ISO
    glutamate metabolic process GO:0006536 ISS
    glutamate catabolic process GO:0006538 ISO
    female pregnancy GO:0007565 IEP
    lactation GO:0007595 IEP
    biosynthetic process GO:0009058 IEA
    response to muscle activity GO:0014850 IEP
    fatty acid transport GO:0015908 ISO
    fatty acid transport GO:0015908 TAS
    glutamate catabolic process to aspartate GO:0019550 ISO
    response to insulin GO:0032868 IEP
    malate-aspartate shuttle GO:0043490 ISO
    dicarboxylic acid metabolic process GO:0043648 IDA
    response to ethanol GO:0045471 ISO
    L-glutamate biosynthetic process GO:0097054 IDA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial matrix GO:0005759 ISO
    mitochondrial matrix GO:0005759 TAS
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IDA
    T-tubule GO:0030315 IDA
    protein-containing complex GO:0032991 IDA
    sarcolemma GO:0042383 IDA
    perikaryon GO:0043204 IDA
 Experiment description of studies that identified Got2 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Got2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Uba52 64156
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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