Gene description for GALNT3
Gene name polypeptide N-acetylgalactosaminyltransferase 3
Gene symbol GALNT3
Other names/aliases GalNAc-T3
HFTC
HHS
Species Homo sapiens
 Database cross references - GALNT3
ExoCarta ExoCarta_2591
Vesiclepedia VP_2591
Entrez Gene 2591
HGNC 4125
MIM 601756
UniProt Q14435  
 GALNT3 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GALNT3
Molecular Function
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IBA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IDA
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IGI
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 IMP
    polypeptide N-acetylgalactosaminyltransferase activity GO:0004653 TAS
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    manganese ion binding GO:0030145 IDA
    carbohydrate binding GO:0030246 IEA
Biological Process
    carbohydrate metabolic process GO:0005975 NAS
    protein O-linked glycosylation GO:0006493 IBA
    protein O-linked glycosylation GO:0006493 IDA
    protein O-linked glycosylation GO:0006493 IMP
    spermatogenesis GO:0007283 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    O-glycan processing GO:0016266 TAS
    protein O-linked glycosylation via serine GO:0018242 IDA
    protein O-linked glycosylation via threonine GO:0018243 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 TAS
    Golgi cisterna membrane GO:0032580 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GALNT3 in sEVs
1
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GALNT3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APPBP2  
Two-hybrid Homo sapiens
2 RSPRY1 89970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PDXDC1 23042
Affinity Capture-MS Homo sapiens
4 ST14 6768
Affinity Capture-MS Homo sapiens
5 NR3C1 2908
Proximity Label-MS Homo sapiens
6 PAPSS1 9061
Affinity Capture-MS Homo sapiens
7 ZCCHC10  
Affinity Capture-MS Homo sapiens
8 SLC39A4 55630
Affinity Capture-MS Homo sapiens
9 DDX58 23586
Affinity Capture-RNA Homo sapiens
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