Gene description for PARS2
Gene name prolyl-tRNA synthetase 2, mitochondrial (putative)
Gene symbol PARS2
Other names/aliases MT-PRORS
proRS
Species Homo sapiens
 Database cross references - PARS2
ExoCarta ExoCarta_25973
Vesiclepedia VP_25973
Entrez Gene 25973
HGNC 30563
MIM 612036
UniProt Q7L3T8  
 PARS2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PARS2
Molecular Function
    proline-tRNA ligase activity GO:0004827 IBA
    ATP binding GO:0005524 IEA
Biological Process
    prolyl-tRNA aminoacylation GO:0006433 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified PARS2 in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AK4 205
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 MECP2 4204
Affinity Capture-MS Homo sapiens
4 HSD17B4 3295
Co-fractionation Homo sapiens
5 ILF3 3609
Co-fractionation Homo sapiens
6 ALDH16A1 126133
Co-fractionation Homo sapiens
7 ICT1 3396
Affinity Capture-MS Homo sapiens
8 IL25  
Affinity Capture-MS Homo sapiens
9 LAMP1 3916
Affinity Capture-MS Homo sapiens
10 PRKAA2 5563
Affinity Capture-MS Homo sapiens
11 DLD 1738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ZC4H2 55906
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 LMX1B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NDUFS7 374291
Affinity Capture-MS Homo sapiens
16 EPRS 2058
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 LARS 51520
Co-fractionation Homo sapiens
18 LPCAT1 79888
Affinity Capture-MS Homo sapiens
19 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
20 LRPPRC 10128
Co-fractionation Homo sapiens
21 BRD1 23774
Affinity Capture-MS Homo sapiens
22 CAPZB 832
Affinity Capture-MS Homo sapiens
23 EMILIN1 11117
Affinity Capture-MS Homo sapiens
24 HSPB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 YARS2  
Affinity Capture-MS Homo sapiens
26 PDHX 8050
Co-fractionation Homo sapiens
27 STIM2 57620
Affinity Capture-MS Homo sapiens
28 FOXM1  
Affinity Capture-MS Homo sapiens
29 XRCC6 2547
Co-fractionation Homo sapiens
30 DSE 29940
Affinity Capture-MS Homo sapiens
31 MRPS24 64951
Affinity Capture-MS Homo sapiens
32 DDRGK1 65992
Affinity Capture-MS Homo sapiens
33 RARS 5917
Co-fractionation Homo sapiens
34 DHX9 1660
Co-fractionation Homo sapiens
35 KRAS 3845
Negative Genetic Homo sapiens
36 DLAT 1737
Co-fractionation Homo sapiens
37 NQO2 4835
Affinity Capture-MS Homo sapiens
38 CORO1B 57175
Co-fractionation Homo sapiens
39 DNAJB11 51726
Affinity Capture-MS Homo sapiens
40 PIN4 5303
Affinity Capture-MS Homo sapiens
41 IGKV1D-13  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PARS2 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Mitochondrial tRNA aminoacylation IEA Reactome
Translation IEA Reactome
tRNA Aminoacylation IEA Reactome





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