Gene description for HIGD1A
Gene name HIG1 hypoxia inducible domain family, member 1A
Gene symbol HIGD1A
Other names/aliases HIG1
RCF1a
Species Homo sapiens
 Database cross references - HIGD1A
ExoCarta ExoCarta_25994
Vesiclepedia VP_25994
Entrez Gene 25994
HGNC 29527
UniProt Q9Y241  
 HIGD1A identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HIGD1A
Biological Process
    negative regulation of apoptotic process GO:0043066 IBA
    negative regulation of apoptotic process GO:0043066 IDA
    mitochondrial respirasome assembly GO:0097250 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified HIGD1A in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HIGD1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ITGA8 8516
Affinity Capture-MS Homo sapiens
2 DUSP19  
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 KLF16  
Affinity Capture-MS Homo sapiens
5 PSMB10 5699
Affinity Capture-MS Homo sapiens
6 ATP1A3 478
Affinity Capture-MS Homo sapiens
7 NR3C1 2908
Proximity Label-MS Homo sapiens
8 SDHA 6389
Co-fractionation Homo sapiens
9 RC3H2  
Affinity Capture-MS Homo sapiens
10 INSIG2  
Affinity Capture-MS Homo sapiens
11 Dync1li1 235661
Affinity Capture-MS Mus musculus
12 PCDHB15  
Affinity Capture-MS Homo sapiens
13 PROZ 8858
Affinity Capture-MS Homo sapiens
14 SERPINB13 5275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
17 SAMD1  
Affinity Capture-MS Homo sapiens
18 POLR3K  
Affinity Capture-MS Homo sapiens
19 STK31  
Affinity Capture-MS Homo sapiens
20 ERGIC1 57222
Co-fractionation Homo sapiens
21 DUSP16  
Affinity Capture-MS Homo sapiens
22 RIPK4  
Affinity Capture-MS Homo sapiens
23 C1orf112  
Affinity Capture-MS Homo sapiens
24 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
25 UFL1 23376
Affinity Capture-MS Homo sapiens
26 CAPZB 832
Affinity Capture-MS Homo sapiens
27 ARMC6 93436
Affinity Capture-MS Homo sapiens
28 MAPK6  
Affinity Capture-MS Homo sapiens
29 MYC  
Affinity Capture-MS Homo sapiens
30 FAM13B  
Affinity Capture-MS Homo sapiens
31 UBE2O 63893
Affinity Capture-MS Homo sapiens
32 Tube1  
Affinity Capture-MS Mus musculus
33 IPO13 9670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 SERBP1 26135
Affinity Capture-MS Homo sapiens
35 Jup 16480
Affinity Capture-MS Mus musculus
36 Kctd5  
Affinity Capture-MS Mus musculus
37 KIAA0368 23392
Affinity Capture-MS Homo sapiens
38 RC3H1 149041
Affinity Capture-MS Homo sapiens
39 PMAIP1  
Affinity Capture-MS Homo sapiens
40 C9orf72  
Affinity Capture-MS Homo sapiens
41 STAG2 10735
Affinity Capture-MS Homo sapiens
42 COMT 1312
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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