Experiment description of studies that identified MOXD1 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 259 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name A375M Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

Protein-protein interactions for MOXD1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ZRANB1 54764 Affinity Capture-MS Homo sapiens 2 C7orf34   Affinity Capture-MS Homo sapiens 3 BAGE2   Affinity Capture-MS Homo sapiens 4 WDR5 11091 Affinity Capture-Western Homo sapiens 5 CSNK2B 1460 Two-hybrid Homo sapiens 6 GPAA1 8733 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 SIAE 54414 Affinity Capture-MS Homo sapiens 8 DEFA1 1667 Affinity Capture-MS Homo sapiens 9 SCGB1D1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 LRRC59 55379 Proximity Label-MS Homo sapiens 11 TAZ   Affinity Capture-MS Homo sapiens 12 TMEM25   Affinity Capture-MS Homo sapiens 13 CHST8 64377 Affinity Capture-MS Homo sapiens 14 PON2 5445 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 FAM19A4   Affinity Capture-MS Homo sapiens 16 CEACAM21   Affinity Capture-MS Homo sapiens 17 SLURP1   Affinity Capture-MS Homo sapiens 18 SLAMF1 6504 Affinity Capture-MS Homo sapiens 19 INSL5   Affinity Capture-MS Homo sapiens 20 B3GNT2 10678 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 SPCS1 28972 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 SPPL2B 56928 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 GPC3 2719 Affinity Capture-MS Homo sapiens 24 PTPRK 5796 Affinity Capture-MS Homo sapiens 25 GLRX3 10539 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 26 KIR2DL4   Affinity Capture-MS Homo sapiens 27 OBP2A 29991 Affinity Capture-MS Homo sapiens 28 SUSD4   Affinity Capture-MS Homo sapiens 29 GPHA2   Affinity Capture-MS Homo sapiens 30 PTCH1   Affinity Capture-MS Homo sapiens 31 CANX 821 Affinity Capture-MS Homo sapiens 32 DEFB109P1B   Affinity Capture-MS Homo sapiens 33 C1QTNF7   Affinity Capture-MS Homo sapiens View the network

Pathways in which MOXD1 is involved

No pathways found