Gene description for RNU5B-1
Gene name RNA, U5B small nuclear 1
Gene symbol RNU5B-1
Other names/aliases RNU5B
RNU5B-1P
U5B1
Species Homo sapiens
 Database cross references - RNU5B-1
ExoCarta ExoCarta_26832
Vesiclepedia VP_26832
Entrez Gene 26832
HGNC 10212
 RNU5B-1 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for RNU5B-1
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 IEA
    formation of quadruple SL/U4/U5/U6 snRNP GO:0000353 IEA
Subcellular Localization
    U5 snRNP GO:0005682 IEA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IEA
 Experiment description of studies that identified RNU5B-1 in sEVs
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule snRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for RNU5B-1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 G3BP1 10146
Affinity Capture-RNA Homo sapiens
2 UBAP2L 9898
Affinity Capture-RNA Homo sapiens
3 ILF3 3609
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which RNU5B-1 is involved
No pathways found





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