Gene description for GCLC
Gene name glutamate-cysteine ligase, catalytic subunit
Gene symbol GCLC
Other names/aliases GCL
GCS
GLCL
GLCLC
Species Homo sapiens
 Database cross references - GCLC
ExoCarta ExoCarta_2729
Vesiclepedia VP_2729
Entrez Gene 2729
HGNC 4311
MIM 606857
UniProt P48506  
 GCLC identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GCLC
Molecular Function
    magnesium ion binding GO:0000287 IDA
    glutamate-cysteine ligase activity GO:0004357 IBA
    glutamate-cysteine ligase activity GO:0004357 IDA
    glutamate-cysteine ligase activity GO:0004357 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    glutamate binding GO:0016595 IDA
    ADP binding GO:0043531 IDA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    cysteine metabolic process GO:0006534 IDA
    glutamate metabolic process GO:0006536 IDA
    glutathione biosynthetic process GO:0006750 IBA
    glutathione biosynthetic process GO:0006750 IDA
    glutathione biosynthetic process GO:0006750 IEA
    glutathione biosynthetic process GO:0006750 IMP
    response to oxidative stress GO:0006979 IDA
    response to nutrient GO:0007584 IEA
    response to heat GO:0009408 IDA
    response to xenobiotic stimulus GO:0009410 IEA
    response to hormone GO:0009725 IDA
    response to activity GO:0014823 IEA
    L-ascorbic acid metabolic process GO:0019852 IEA
    negative regulation of protein ubiquitination GO:0031397 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    cellular response to hepatocyte growth factor stimulus GO:0035729 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    cellular response to fibroblast growth factor stimulus GO:0044344 IEA
    response to human chorionic gonadotropin GO:0044752 IEA
    cell redox homeostasis GO:0045454 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    response to arsenic-containing substance GO:0046685 IEA
    response to cadmium ion GO:0046686 IEA
    response to nitrosative stress GO:0051409 IEA
    regulation of mitochondrial depolarization GO:0051900 IEA
    response to interleukin-1 GO:0070555 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to follicle-stimulating hormone stimulus GO:0071372 IEA
    cellular response to thyroxine stimulus GO:0097069 IEA
    blood vessel diameter maintenance GO:0097746 IMP
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 IEA
    negative regulation of hepatic stellate cell activation GO:2000490 IEA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 TAS
    glutamate-cysteine ligase complex GO:0017109 IBA
    glutamate-cysteine ligase complex GO:0017109 IDA
 Experiment description of studies that identified GCLC in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GCLC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 HNRNPDL 9987
Co-fractionation Homo sapiens
3 ZRANB1 54764
Affinity Capture-MS Homo sapiens
4 AGL 178
Co-fractionation Homo sapiens
5 CCL22 6367
Two-hybrid Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 SSR3 6747
Cross-Linking-MS (XL-MS) Homo sapiens
8 PAXIP1  
Affinity Capture-MS Homo sapiens
9 FEN1 2237
Cross-Linking-MS (XL-MS) Homo sapiens
10 RPA2 6118
Proximity Label-MS Homo sapiens
11 PPID 5481
Co-fractionation Homo sapiens
12 CUL3 8452
Affinity Capture-MS Homo sapiens
13 TYW3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 NAA50 80218
Co-fractionation Homo sapiens
15 GCLM 2730
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 PARK2  
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 RPA3 6119
Proximity Label-MS Homo sapiens
20 PAK4 10298
Co-fractionation Homo sapiens
21 DPH5 51611
Co-fractionation Homo sapiens
22 NMD3 51068
Affinity Capture-MS Homo sapiens
23 DHRS11 79154
Co-fractionation Homo sapiens
24 PRR16  
Affinity Capture-MS Homo sapiens
25 CUL4A 8451
Affinity Capture-MS Homo sapiens
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