Gene description for GNL1
Gene name guanine nucleotide binding protein-like 1
Gene symbol GNL1
Other names/aliases HSR1
Species Homo sapiens
 Database cross references - GNL1
ExoCarta ExoCarta_2794
Vesiclepedia VP_2794
Entrez Gene 2794
HGNC 4413
MIM 143024
UniProt P36915  
 GNL1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GNL1
Molecular Function
    GTPase activity GO:0003924 IBA
    structural molecule activity GO:0005198 NAS
    GTP binding GO:0005525 NAS
Biological Process
    T cell mediated immunity GO:0002456 NAS
    DNA damage response GO:0006974 IDA
    signal transduction GO:0007165 NAS
Subcellular Localization
    extracellular space GO:0005615 NAS
    nucleus GO:0005634 HDA
 Experiment description of studies that identified GNL1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GNL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX18 8886
Co-fractionation Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GTF2F1 2962
Co-fractionation Homo sapiens
5 HNRNPC 3183
Co-fractionation Homo sapiens
6 PPP6R3 55291
Co-fractionation Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 MIER1  
Co-fractionation Homo sapiens
9 ALDOC 230
Co-fractionation Homo sapiens
10 KPNA3 3839
Co-fractionation Homo sapiens
11 NOP58 51602
Co-fractionation Homo sapiens
12 NOLC1 9221
Co-fractionation Homo sapiens
13 PABPC4 8761
Co-fractionation Homo sapiens
14 EIF5B 9669
Co-fractionation Homo sapiens
15 AHCYL1 10768
Co-fractionation Homo sapiens
16 DNAJB6 10049
Affinity Capture-MS Homo sapiens
17 PES1 23481
Co-fractionation Homo sapiens
18 CCDC116  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RUVBL2 10856
Co-fractionation Homo sapiens
20 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 USP9X 8239
Co-fractionation Homo sapiens
22 UBR4 23352
Co-fractionation Homo sapiens
23 TCOF1 6949
Co-fractionation Homo sapiens
24 TUBB4B 10383
Co-fractionation Homo sapiens
25 SIRPA 140885
Reconstituted Complex Homo sapiens
26 HINT1 3094
Affinity Capture-MS Homo sapiens
27 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PRPF8 10594
Co-fractionation Homo sapiens
29 NTNG1  
Affinity Capture-MS Homo sapiens
30 MRPL42  
Affinity Capture-MS Homo sapiens
31 PTP4A1 7803
Two-hybrid Homo sapiens
32 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
33 CTDP1 9150
Co-fractionation Homo sapiens
34 RPL4 6124
Affinity Capture-MS Homo sapiens
35 Mical3  
Affinity Capture-MS Mus musculus
36 KLHL40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 RPL7 6129
Co-fractionation Homo sapiens
38 SETDB1  
Co-fractionation Homo sapiens
39 KPNB1 3837
Co-fractionation Homo sapiens
40 RPL5 6125
Co-fractionation Homo sapiens
41 ZNF212  
Two-hybrid Homo sapiens
42 KRR1 11103
Co-fractionation Homo sapiens
43 PARK2  
Affinity Capture-MS Homo sapiens
44 ABCF2 10061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PGK1 5230
Affinity Capture-MS Homo sapiens
46 NCL 4691
Co-fractionation Homo sapiens
47 ALYREF 10189
Co-fractionation Homo sapiens
48 NRD1 4898
Co-fractionation Homo sapiens
49 RPA3 6119
Proximity Label-MS Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 PAPSS1 9061
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
53 EP300 2033
Affinity Capture-MS Homo sapiens
54 EIF3B 8662
Co-fractionation Homo sapiens
55 CSNK2B 1460
Affinity Capture-MS Homo sapiens
56 C1QBP 708
Proximity Label-MS Homo sapiens
57 HUWE1 10075
Co-fractionation Homo sapiens
58 GDI1 2664
Two-hybrid Homo sapiens
59 KIF1BP 26128
Co-fractionation Homo sapiens
60 C2orf44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RPLP0 6175
Co-fractionation Homo sapiens
62 SRP14 6727
Affinity Capture-MS Homo sapiens
63 RPS15A 6210
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
64 GNB2L1 10399
Affinity Capture-MS Homo sapiens
65 RANGAP1 5905
Co-fractionation Homo sapiens
66 RAD21 5885
Affinity Capture-Western Homo sapiens
67 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 RPL19 6143
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GNL1 is involved
No pathways found





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