Experiment description of studies that identified SLC43A3 in sEVs

1 Experiment ID 79 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

2 Experiment ID 80 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

3 Experiment ID 81 MISEV standards ✘ Biophysical techniques ✔ CD81|MHCII Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W." Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

4 Experiment ID 258 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name SKMEL28 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 260 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for SLC43A3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SIGMAR1 10280 Affinity Capture-MS Homo sapiens 2 LAMC3   Two-hybrid Homo sapiens 3 ATP13A3 79572 Affinity Capture-MS Homo sapiens 4 Ercc6l   Affinity Capture-MS Mus musculus 5 KDELR1 10945 Affinity Capture-MS Homo sapiens 6 NASP 4678 Affinity Capture-MS Homo sapiens 7 ATP5J 522 Affinity Capture-MS Homo sapiens 8 SYNGR1 9145 Affinity Capture-MS Homo sapiens 9 Mad2l1bp   Affinity Capture-MS Mus musculus 10 AP1S2 8905 Affinity Capture-MS Homo sapiens 11 ENDOD1 23052 Affinity Capture-MS Homo sapiens 12 TTR 7276 Affinity Capture-MS Homo sapiens 13 MBOAT1   Affinity Capture-MS Homo sapiens 14 LIMCH1 22998 Affinity Capture-MS Homo sapiens 15 UBA52 7311 Affinity Capture-MS Homo sapiens 16 Atp2a2 11938 Affinity Capture-MS Mus musculus 17 TSPAN15 23555 Affinity Capture-MS Homo sapiens 18 Hspa14 50497 Affinity Capture-MS Mus musculus 19 Timeless   Affinity Capture-MS Mus musculus 20 LGALS3 3958 Affinity Capture-MS Homo sapiens 21 MFSD8 256471 Affinity Capture-MS Homo sapiens 22 DDX58 23586 Affinity Capture-RNA Homo sapiens 23 Ndel1   Affinity Capture-MS Mus musculus 24 IFITM1 8519 Affinity Capture-MS Homo sapiens 25 MCAM 4162 Proximity Label-MS Homo sapiens 26 RABL3 285282 Affinity Capture-MS Homo sapiens 27 ARL6IP6   Affinity Capture-MS Homo sapiens 28 Kcnk1   Affinity Capture-MS Mus musculus 29 DAB2 1601 Affinity Capture-MS Homo sapiens 30 STRN 6801 Affinity Capture-MS Homo sapiens 31 SPPL2B 56928 Affinity Capture-MS Homo sapiens 32 CIT 11113 Affinity Capture-MS Homo sapiens 33 APOB 338 Affinity Capture-MS Homo sapiens 34 CHST15 51363 Affinity Capture-MS Homo sapiens 35 ATP5C1 509 Affinity Capture-MS Homo sapiens 36 VMA21   Affinity Capture-MS Homo sapiens 37 HTR1B   Affinity Capture-MS Homo sapiens 38 ATP11C 286410 Affinity Capture-MS Homo sapiens 39 ATP5B 506 Affinity Capture-MS Homo sapiens 40 SURF4 6836 Affinity Capture-MS Homo sapiens 41 KRBOX4   Affinity Capture-MS Homo sapiens View the network

Pathways in which SLC43A3 is involved

No pathways found