Experiment description of studies that identified Tufm in sEVs

1 Experiment ID 225 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✔ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25998041     Organism Rattus norvegicus Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. Authors "Lee JE, Moon PG, Lee IK, Baek MC" Journal name Protein J Publication year 2015 Sample Adipocytes Sample name Adipocytes Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

2 Experiment ID 97 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Tufm

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Dao   Affinity Capture-MS Rattus norvegicus 2 STXBP5L   Affinity Capture-MS Homo sapiens 3 Itm2b 290364 Affinity Capture-MS Rattus norvegicus View the network

Pathways in which Tufm is involved

No pathways found