Gene description for Scamp1
Gene name secretory carrier membrane protein 1
Gene symbol Scamp1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Scamp1
ExoCarta ExoCarta_29521
Vesiclepedia VP_29521
Entrez Gene 29521
UniProt P56603  
 Scamp1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Scamp1
Molecular Function
    protein domain specific binding GO:0019904 IDA
Biological Process
    exocytosis GO:0006887 IEA
    exocytosis GO:0006887 ISO
    endocytosis GO:0006897 IDA
    protein transport GO:0015031 IBA
    protein transport GO:0015031 IEA
    protein transport GO:0015031 ISO
    protein transport GO:0015031 ISS
    establishment of localization in cell GO:0051649 IEA
    establishment of localization in cell GO:0051649 ISO
Subcellular Localization
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    clathrin-coated vesicle GO:0030136 IDA
    cytoplasmic vesicle membrane GO:0030659 ISO
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 ISO
    trans-Golgi network membrane GO:0032588 IBA
    zymogen granule membrane GO:0042589 IEA
    zymogen granule membrane GO:0042589 ISO
    terminal bouton GO:0043195 HDA
    synapse GO:0045202 ISO
    recycling endosome membrane GO:0055038 IBA
    recycling endosome membrane GO:0055038 IEA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
 Experiment description of studies that identified Scamp1 in sEVs
1
Experiment ID 90
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Scamp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Scamp1 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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