Gene description for Pdha1
Gene name pyruvate dehydrogenase (lipoamide) alpha 1
Gene symbol Pdha1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Pdha1
ExoCarta ExoCarta_29554
Vesiclepedia VP_29554
Entrez Gene 29554
 Pdha1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Pdha1
Molecular Function
    pyruvate dehydrogenase activity GO:0004738 ISO
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 IDA
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 IEA
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 ISO
    pyruvate dehydrogenase (acetyl-transferring) activity GO:0004739 ISS
    pyruvate dehydrogenase (NAD+) activity GO:0034604 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    glucose metabolic process GO:0006006 IEA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IBA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IDA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 IEA
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    acetyl-CoA biosynthetic process from pyruvate GO:0006086 ISO
    tricarboxylic acid cycle GO:0006099 IEA
Subcellular Localization
    nucleolus GO:0005730 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    intracellular membrane-bounded organelle GO:0043231 IEA
    pyruvate dehydrogenase complex GO:0045254 IDA
    pyruvate dehydrogenase complex GO:0045254 ISO
    catalytic complex GO:1902494 ISO
 Experiment description of studies that identified Pdha1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Pdha1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Pdha1 is involved
No pathways found





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