Gene description for Ywhae
Gene name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Gene symbol Ywhae
Other names/aliases 14-3-3e
Species Rattus norvegicus
 Database cross references - Ywhae
ExoCarta ExoCarta_29753
Vesiclepedia VP_29753
Entrez Gene 29753
UniProt P62260  
 Ywhae identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Ywhae
Molecular Function
    protein phosphatase inhibitor activity GO:0004864 ISO
    calcium channel regulator activity GO:0005246 ISO
    protein binding GO:0005515 IPI
    protein kinase C inhibitor activity GO:0008426 TAS
    potassium channel regulator activity GO:0015459 ISO
    calcium channel inhibitor activity GO:0019855 ISO
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    signaling adaptor activity GO:0035591 ISO
    identical protein binding GO:0042802 ISO
    histone deacetylase binding GO:0042826 ISO
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 IPI
    protein heterodimerization activity GO:0046982 ISO
    phosphoserine residue binding GO:0050815 IBA
    phosphoserine residue binding GO:0050815 ISO
    phosphoprotein binding GO:0051219 ISO
    scaffold protein binding GO:0097110 ISO
    protein sequestering activity GO:0140311 ISO
Biological Process
    MAPK cascade GO:0000165 ISO
    MAPK cascade GO:0000165 ISS
    neuron migration GO:0001764 IEA
    neuron migration GO:0001764 ISO
    cytoplasmic pattern recognition receptor signaling pathway GO:0002753 ISO
    protein targeting GO:0006605 IEA
    protein targeting GO:0006605 ISO
    signal transduction GO:0007165 IBA
    regulation of mitotic cell cycle GO:0007346 IBA
    protein localization GO:0008104 IBA
    substantia nigra development GO:0021762 ISO
    hippocampus development GO:0021766 IEA
    hippocampus development GO:0021766 ISO
    cerebral cortex development GO:0021987 IEA
    cerebral cortex development GO:0021987 ISO
    negative regulation of toll-like receptor signaling pathway GO:0034122 ISO
    protein localization to nucleus GO:0034504 ISO
    protein localization to nucleus GO:0034504 ISS
    cellular response to heat GO:0034605 ISO
    cellular response to heat GO:0034605 ISS
    positive regulation of hippo signaling GO:0035332 ISO
    positive regulation of protein export from nucleus GO:0046827 ISO
    positive regulation of protein export from nucleus GO:0046827 ISS
    cytoplasmic sequestering of protein GO:0051220 ISO
    regulation of cytosolic calcium ion concentration GO:0051480 ISO
    regulation of membrane repolarization GO:0060306 ISO
    protein localization to endoplasmic reticulum GO:0070972 ISO
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 EXP
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IDA
    regulation of potassium ion transmembrane transport GO:1901379 ISO
    negative regulation of calcium ion export across plasma membrane GO:1905913 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    kinesin complex GO:0005871 IDA
    plasma membrane GO:0005886 ISO
    axon GO:0030424 IDA
    melanosome GO:0042470 IEA
    synapse GO:0045202 IDA
    central region of growth cone GO:0090724 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Ywhae in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
11
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
12
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ywhae
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmd4  
Affinity Capture-Western Rattus norvegicus
2 Cdc25b  
Affinity Capture-Western Rattus norvegicus
3 Cdc25a  
Affinity Capture-Western Rattus norvegicus
4 Map3k3  
Two-hybrid Rattus norvegicus
5 Cdc20  
Co-fractionation Rattus norvegicus
6 HDAC5 10014
Affinity Capture-Western Homo sapiens
7 Cdc25c  
Affinity Capture-Western Rattus norvegicus
8 Nedd4 25489
Affinity Capture-Western Rattus norvegicus
9 Clic4 83718
Affinity Capture-MS Rattus norvegicus
10 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ywhae is involved
PathwayEvidenceSource
Activation of BAD and translocation to mitochondria IEA Reactome
Activation of BH3-only proteins IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Apoptosis IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cell death signalling via NRAGE, NRIF and NADE IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex IEA Reactome
Cilium Assembly IEA Reactome
Death Receptor Signaling IEA Reactome
G2/M Checkpoints IEA Reactome
G2/M DNA damage checkpoint IEA Reactome
G2/M Transition IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
HSF1 activation IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
NADE modulates death signalling IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
p75 NTR receptor-mediated signalling IEA Reactome
Programmed Cell Death IEA Reactome
RAB GEFs exchange GTP for GDP on RABs IEA Reactome
Rab regulation of trafficking IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases activate PKNs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Signal Transduction IEA Reactome
Signaling by Hippo IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
TP53 Regulates Metabolic Genes IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome
Vesicle-mediated transport IEA Reactome





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