Gene description for Atp6v0a1
Gene name ATPase, H+ transporting, lysosomal V0 subunit A1
Gene symbol Atp6v0a1
Other names/aliases Atp6n1
Atp6n1a
Species Rattus norvegicus
 Database cross references - Atp6v0a1
ExoCarta ExoCarta_29757
Vesiclepedia VP_29757
Entrez Gene 29757
UniProt P25286  
 Atp6v0a1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Atp6v0a1
Molecular Function
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ATPase binding GO:0051117 IBA
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
Biological Process
    vacuolar acidification GO:0007035 IBA
    biological_process GO:0008150 ND
    regulation of macroautophagy GO:0016241 IEA
    regulation of macroautophagy GO:0016241 ISO
    synaptic vesicle lumen acidification GO:0097401 IEA
    synaptic vesicle lumen acidification GO:0097401 ISO
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 IEA
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 ISS
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IBA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    clathrin-coated vesicle membrane GO:0030665 IEA
    synaptic vesicle membrane GO:0030672 EXP
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IEA
    melanosome GO:0042470 IEA
    terminal bouton GO:0043195 HDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Atp6v0a1 in sEVs
1
Experiment ID 99
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 100
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp6v0a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 STXBP5L  
Affinity Capture-MS Homo sapiens
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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