Gene description for Dlst
Gene name dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Gene symbol Dlst
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dlst
ExoCarta ExoCarta_299201
Vesiclepedia VP_299201
Entrez Gene 299201
UniProt Q01205  
 Dlst identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dlst
Molecular Function
    dihydrolipoyllysine-residue succinyltransferase activity GO:0004149 IBA
    dihydrolipoyllysine-residue succinyltransferase activity GO:0004149 IDA
    dihydrolipoyllysine-residue succinyltransferase activity GO:0004149 IEA
    dihydrolipoyllysine-residue succinyltransferase activity GO:0004149 ISO
    acyltransferase activity GO:0016746 IEA
    acyltransferase activity GO:0016746 ISO
    heat shock protein binding GO:0031072 IPI
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    tricarboxylic acid cycle GO:0006099 IBA
    tricarboxylic acid cycle GO:0006099 IC
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 ISO
    tricarboxylic acid cycle GO:0006099 ISS
    2-oxoglutarate metabolic process GO:0006103 IDA
    2-oxoglutarate metabolic process GO:0006103 ISO
    succinyl-CoA metabolic process GO:0006104 ISO
    succinyl-CoA metabolic process GO:0006104 ISS
    NADH metabolic process GO:0006734 IDA
    carboxylic acid metabolic process GO:0019752 IEA
    L-lysine catabolic process to acetyl-CoA via saccharopine GO:0033512 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    oxoglutarate dehydrogenase complex GO:0045252 IDA
    oxoglutarate dehydrogenase complex GO:0045252 IEA
    oxoglutarate dehydrogenase complex GO:0045252 ISO
    oxoadipate dehydrogenase complex GO:0160167 IEA
    oxoadipate dehydrogenase complex GO:0160167 ISO
    catalytic complex GO:1902494 IEA
 Experiment description of studies that identified Dlst in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dlst
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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