Gene description for IFI35
Gene name interferon-induced protein 35
Gene symbol IFI35
Other names/aliases IFP35
Species Homo sapiens
 Database cross references - IFI35
ExoCarta ExoCarta_3430
Vesiclepedia VP_3430
Entrez Gene 3430
HGNC 5399
MIM 600735
UniProt P80217  
 IFI35 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IFI35
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IDA
Biological Process
    macrophage activation involved in immune response GO:0002281 IDA
    negative regulation of cell population proliferation GO:0008285 IMP
    positive regulation of toll-like receptor 4 signaling pathway GO:0034145 IDA
    innate immune response GO:0045087 IEA
    positive regulation of innate immune response GO:0045089 IDA
    positive regulation of inflammatory response GO:0050729 IDA
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 IMP
    positive regulation of non-canonical NF-kappaB signal transduction GO:1901224 ISS
Subcellular Localization
    extracellular space GO:0005615 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified IFI35 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IFI35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Two-hybrid Homo sapiens
2 CLEC4G  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 SHKBP1  
Affinity Capture-MS Homo sapiens
4 TRIM21 6737
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 TAB1 10454
Affinity Capture-MS Homo sapiens
6 PLXNA1 5361
Affinity Capture-MS Homo sapiens
7 TRIM7  
Affinity Capture-MS Homo sapiens
8 IFT122 55764
Affinity Capture-MS Homo sapiens
9 KDM1A 23028
Two-hybrid Homo sapiens
10 HAUS5 23354
Affinity Capture-MS Homo sapiens
11 NR3C1 2908
Proximity Label-MS Homo sapiens
12 BATF  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
13 NFRKB  
Affinity Capture-MS Homo sapiens
14 PHF19  
Affinity Capture-MS Homo sapiens
15 PTBP3 9991
Affinity Capture-MS Homo sapiens
16 TRIM55  
Two-hybrid Homo sapiens
17 PLEKHO1 51177
Two-hybrid Homo sapiens
18 NMI  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
19 WDR81 124997
Affinity Capture-MS Homo sapiens
20 CYTH3 9265
Affinity Capture-MS Homo sapiens
21 TRIM63  
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here