Gene description for IGFALS
Gene name insulin-like growth factor binding protein, acid labile subunit
Gene symbol IGFALS
Other names/aliases ACLSD
ALS
Species Homo sapiens
 Database cross references - IGFALS
ExoCarta ExoCarta_3483
Vesiclepedia VP_3483
Entrez Gene 3483
HGNC 5468
MIM 601489
UniProt P35858  
 IGFALS identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Urine 19056867    
 Gene ontology annotations for IGFALS
Molecular Function
    insulin-like growth factor binding GO:0005520 IBA
Biological Process
    cell adhesion GO:0007155 IEA
    signal transduction GO:0007165 TAS
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular matrix GO:0031012 IBA
    insulin-like growth factor ternary complex GO:0042567 IBA
    insulin-like growth factor ternary complex GO:0042567 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified IGFALS in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for IGFALS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA5 3309
Affinity Capture-MS Homo sapiens
2 SEL1L 6400
Affinity Capture-MS Homo sapiens
3 ARID1A 8289
Negative Genetic Homo sapiens
4 ARRB2 409
Co-fractionation Homo sapiens
5 GOLGA6L2  
Affinity Capture-MS Homo sapiens
6 ZBTB33  
Affinity Capture-MS Homo sapiens
7 IGF1  
Affinity Capture-Western Homo sapiens
8 CNPY3 10695
Affinity Capture-MS Homo sapiens
9 VHL  
Negative Genetic Homo sapiens
10 IGFBP5 3488
Reconstituted Complex Homo sapiens
11 WDR25  
Affinity Capture-MS Homo sapiens
12 IGFBP3 3486
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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