Gene description for INCENP
Gene name inner centromere protein antigens 135/155kDa
Gene symbol INCENP
Other names/aliases -
Species Homo sapiens
 Database cross references - INCENP
ExoCarta ExoCarta_3619
Vesiclepedia VP_3619
Entrez Gene 3619
HGNC 6058
MIM 604411
UniProt Q9NQS7  
 INCENP identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for INCENP
Molecular Function
    protein binding GO:0005515 IPI
    molecular function activator activity GO:0140677 IPI
Biological Process
    mitotic cell cycle GO:0000278 NAS
    mitotic cytokinesis GO:0000281 IBA
    mitotic cytokinesis GO:0000281 IMP
    mitotic cytokinesis GO:0000281 NAS
    chromosome segregation GO:0007059 IMP
    mitotic spindle midzone assembly GO:0051256 NAS
    meiotic spindle midzone assembly GO:0051257 IBA
    metaphase chromosome alignment GO:0051310 IBA
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 NAS
    mitotic spindle assembly GO:0090307 NAS
    positive regulation of mitotic sister chromatid separation GO:1901970 NAS
    positive regulation of attachment of mitotic spindle microtubules to kinetochore GO:1902425 NAS
    positive regulation of mitotic cytokinesis GO:1903490 NAS
Subcellular Localization
    chromosome, centromeric region GO:0000775 IDA
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IDA
    lateral element GO:0000800 IEA
    central element GO:0000801 IEA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    pericentric heterochromatin GO:0005721 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    chromocenter GO:0010369 IEA
    microtubule cytoskeleton GO:0015630 IDA
    nuclear body GO:0016604 IDA
    midbody GO:0030496 IBA
    midbody GO:0030496 IDA
    chromosome passenger complex GO:0032133 IBA
    chromosome passenger complex GO:0032133 IPI
    protein-containing complex GO:0032991 IDA
    meiotic spindle midzone GO:1990385 IBA
 Experiment description of studies that identified INCENP in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for INCENP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 Cbx1  
Affinity Capture-MS Mus musculus
4 CDCA8 55143
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
5 Incenp  
Affinity Capture-MS Mus musculus
6 PRKAA1 5562
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 ZBTB2 57621
Affinity Capture-MS Homo sapiens
9 ASF1A 25842
Proximity Label-MS Homo sapiens
10 TP53 7157
Affinity Capture-MS Homo sapiens
11 HIST1H3A 8350
Proximity Label-MS Homo sapiens
12 FBXL6  
Proximity Label-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 ANLN 54443
Affinity Capture-MS Homo sapiens
15 HIST2H3C 126961
Reconstituted Complex Homo sapiens
16 ECT2 1894
Affinity Capture-MS Homo sapiens
17 SIRT7  
Affinity Capture-MS Homo sapiens
18 Nfyc 18046
Affinity Capture-MS Mus musculus
19 AURKA 6790
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 APEX1 328
Proximity Label-MS Homo sapiens
21 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
22 AURKC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HMGN5 79366
Affinity Capture-MS Homo sapiens
24 LMNA 4000
Proximity Label-MS Homo sapiens
25 SGOL1  
Affinity Capture-Western Homo sapiens
26 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
27 EIF2B5 8893
Affinity Capture-MS Homo sapiens
28 TUBA1A 404053
Co-fractionation Bos taurus
29 LYN 4067
Proximity Label-MS Homo sapiens
30 CENPA  
Proximity Label-MS Homo sapiens
31 EVI5  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 AURKB 9212
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 CBX3 11335
Proximity Label-MS Homo sapiens
34 KIF20A 10112
Affinity Capture-MS Homo sapiens
35 Aurkb  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
36 CHMP4C 92421
Affinity Capture-MS Homo sapiens
37 CHMP4B 128866
Affinity Capture-MS Homo sapiens
38 HECW2  
Affinity Capture-MS Homo sapiens
39 H2AFZ 3015
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
40 HECTD1 25831
Affinity Capture-MS Homo sapiens
41 KIF23 9493
Affinity Capture-MS Homo sapiens
42 CBX5 23468
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
43 S100P 6286
Affinity Capture-MS Homo sapiens
44 SLX4  
Affinity Capture-MS Homo sapiens
45 TOLLIP 54472
Proximity Label-MS Homo sapiens
46 C1QBP 708
Proximity Label-MS Homo sapiens
47 PRC1 9055
Affinity Capture-MS Homo sapiens
48 Cdca8  
Affinity Capture-MS Mus musculus
49 PARP1 142
Proximity Label-MS Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 PHF8  
Affinity Capture-MS Homo sapiens
52 FLOT1 10211
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
53 BIRC5  
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
54 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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