Gene description for KCND3
Gene name potassium channel, voltage gated Shal related subfamily D, member 3
Gene symbol KCND3
Other names/aliases KCND3L
KCND3S
KSHIVB
KV4.3
SCA19
SCA22
Species Homo sapiens
 Database cross references - KCND3
ExoCarta ExoCarta_3752
Vesiclepedia VP_3752
Entrez Gene 3752
HGNC 6239
MIM 605411
UniProt Q9UK17  
 KCND3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for KCND3
Molecular Function
    A-type (transient outward) potassium channel activity GO:0005250 IDA
    A-type (transient outward) potassium channel activity GO:0005250 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    voltage-gated potassium channel activity involved in cardiac muscle cell action potential repolarization GO:0086008 TAS
    voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization GO:1902282 IMP
Biological Process
    action potential GO:0001508 IBA
    potassium ion transport GO:0006813 IDA
    muscle contraction GO:0006936 NAS
    chemical synaptic transmission GO:0007268 NAS
    regulation of heart contraction GO:0008016 NAS
    protein homooligomerization GO:0051260 IEA
    protein tetramerization GO:0051262 IDA
    potassium ion transmembrane transport GO:0071805 IDA
    membrane repolarization GO:0086009 IDA
    membrane repolarization GO:0086009 NAS
    membrane repolarization during cardiac muscle cell action potential GO:0086013 TAS
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    potassium ion export across plasma membrane GO:0097623 IBA
    potassium ion export across plasma membrane GO:0097623 IDA
    potassium ion export across plasma membrane GO:0097623 ISS
    potassium ion export across plasma membrane GO:0097623 NAS
    membrane repolarization during ventricular cardiac muscle cell action potential GO:0098915 IEA
    ventricular cardiac muscle cell membrane repolarization GO:0099625 IMP
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    voltage-gated potassium channel complex GO:0008076 IBA
    voltage-gated potassium channel complex GO:0008076 IDA
    sarcolemma GO:0042383 IEA
    neuronal cell body GO:0043025 IBA
    dendritic spine GO:0043197 IBA
    postsynaptic membrane GO:0045211 IBA
    Kv4.3-KChIP1 channel complex GO:0071196 IPI
    GABA-ergic synapse GO:0098982 IEA
    postsynaptic specialization membrane GO:0099634 IEA
 Experiment description of studies that identified KCND3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KCND3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNBP 7555
Affinity Capture-Western Homo sapiens
2 KCNIP2  
Affinity Capture-Western Homo sapiens
3 SEMA3A  
Affinity Capture-Western Homo sapiens
4 HSPA5 3309
Affinity Capture-Western Homo sapiens
5 TUBB2A 7280
Affinity Capture-Western Homo sapiens
6 Kcnip2  
Affinity Capture-Western Rattus norvegicus
7 LBR 3930
Affinity Capture-Western Homo sapiens
8 HSPA8 3312
Affinity Capture-Western Homo sapiens
9 UQCRC2 7385
Affinity Capture-Western Homo sapiens
10 Rapgef5  
Affinity Capture-Western Rattus norvegicus
11 KCNIP1  
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
12 G3BP1 10146
Affinity Capture-Western Homo sapiens
13 EMD 2010
Affinity Capture-Western Homo sapiens
14 DPP10 57628
Affinity Capture-Western Homo sapiens
15 NAP1L1 4673
Affinity Capture-Western Homo sapiens
16 PYCR2 29920
Affinity Capture-Western Homo sapiens
17 NDUFS2 4720
Affinity Capture-Western Homo sapiens
18 CDK1 983
Affinity Capture-Western Homo sapiens
19 NONO 4841
Affinity Capture-Western Homo sapiens
20 AUP1 550
Affinity Capture-Western Homo sapiens
21 Rapgef4  
Affinity Capture-Western Rattus norvegicus
22 RUVBL1 8607
Affinity Capture-Western Homo sapiens
23 TUBA1B 10376
Affinity Capture-Western Homo sapiens
24 RAPGEF5  
Affinity Capture-Western Homo sapiens
25 Kcnip1  
Affinity Capture-Western Rattus norvegicus
26 HNRNPF 3185
Affinity Capture-Western Homo sapiens
27 XRCC6 2547
Affinity Capture-Western Homo sapiens
28 DDB1 1642
Affinity Capture-Western Homo sapiens
29 RCN2 5955
Affinity Capture-Western Homo sapiens
30 CUX1 1523
Affinity Capture-Western Homo sapiens
31 SRC 6714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 Rapgef3  
Affinity Capture-Western Rattus norvegicus
33 EWSR1 2130
Affinity Capture-Western Homo sapiens
34 XRCC5 7520
Affinity Capture-Western Homo sapiens
35 VPS4A 27183
Affinity Capture-Western Homo sapiens
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