Gene description for BLOC1S3
Gene name biogenesis of lysosomal organelles complex-1, subunit 3
Gene symbol BLOC1S3
Other names/aliases BLOS3
HPS8
RP
Species Homo sapiens
 Database cross references - BLOC1S3
ExoCarta ExoCarta_388552
Vesiclepedia VP_388552
Entrez Gene 388552
HGNC 20914
MIM 609762
UniProt Q6QNY0  
 BLOC1S3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for BLOC1S3
Molecular Function
    protein binding GO:0005515 IPI
    protein transmembrane transporter activity GO:0008320 IEA
Biological Process
    eye development GO:0001654 IMP
    anterograde axonal transport GO:0008089 ISS
    response to xenobiotic stimulus GO:0009410 IEA
    platelet activation GO:0030168 IMP
    neuron projection development GO:0031175 ISS
    melanosome transport GO:0032402 IDA
    melanosome organization GO:0032438 NAS
    positive regulation of natural killer cell activation GO:0032816 IEA
    secretion of lysosomal enzymes GO:0033299 IEA
    endosome to melanosome transport GO:0035646 IDA
    pigmentation GO:0043473 IMP
    anterograde synaptic vesicle transport GO:0048490 ISS
    platelet dense granule organization GO:0060155 NAS
    protein transmembrane transport GO:0071806 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    transport vesicle GO:0030133 IDA
    BLOC-1 complex GO:0031083 IBA
    BLOC-1 complex GO:0031083 IDA
    BLOC-1 complex GO:0031083 IPI
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified BLOC1S3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for BLOC1S3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG9A 79065
Proximity Label-MS Homo sapiens
2 S100A2 6273
Affinity Capture-MS Homo sapiens
3 BLOC1S1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 DTNBP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DNALI1  
Affinity Capture-MS Homo sapiens
6 BLOC1S5 63915
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 BLOC1S6  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 MBLAC2 153364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BLOC1S2 282991
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 ENTHD1  
Affinity Capture-MS Homo sapiens
11 SNAPIN 23557
Affinity Capture-Western Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 BCAS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HSPA2 3306
Affinity Capture-MS Homo sapiens
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