Gene description for STS
Gene name steroid sulfatase (microsomal), isozyme S
Gene symbol STS
Other names/aliases ARSC
ARSC1
ASC
ES
SSDD
XLI
Species Homo sapiens
 Database cross references - STS
ExoCarta ExoCarta_412
Vesiclepedia VP_412
Entrez Gene 412
HGNC 11425
MIM 300747
UniProt P08842  
 STS identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for STS
Molecular Function
    arylsulfatase activity GO:0004065 IBA
    steryl-sulfatase activity GO:0004773 IEA
    sulfuric ester hydrolase activity GO:0008484 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    steroid catabolic process GO:0006706 TAS
    female pregnancy GO:0007565 IEA
    epidermis development GO:0008544 TAS
Subcellular Localization
    lysosome GO:0005764 TAS
    endosome GO:0005768 TAS
    endoplasmic reticulum GO:0005783 TAS
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    intracellular membrane-bounded organelle GO:0043231 TAS
 Experiment description of studies that identified STS in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for STS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC8A 56262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MBOAT7 79143
Affinity Capture-MS Homo sapiens
3 TSPAN16  
Affinity Capture-MS Homo sapiens
4 WNT8A  
Affinity Capture-MS Homo sapiens
5 ABCB8 11194
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FZD2 2535
Affinity Capture-MS Homo sapiens
7 SIDT2 51092
Affinity Capture-MS Homo sapiens
8 TMEM214 54867
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 GJA1 2697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 FUT8 2530
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LMBR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LYZL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
15 CLEC12B  
Affinity Capture-MS Homo sapiens
16 SLC30A1 7779
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ZDHHC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GOLGA5 9950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SCGB2A2  
Affinity Capture-MS Homo sapiens
20 GPHN 10243
Affinity Capture-MS Homo sapiens
21 FAM134A 79137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LMF2 91289
Affinity Capture-MS Homo sapiens
23 JAG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CISD2 493856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SUMF1 285362
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ATP11C 286410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CKAP4 10970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CCR1  
Affinity Capture-MS Homo sapiens
29 ALG9 79796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ASCL1  
Affinity Capture-MS Homo sapiens
31 CLRN2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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