Gene description for MT1G
Gene name metallothionein 1G
Gene symbol MT1G
Other names/aliases MT1
MT1K
Species Homo sapiens
 Database cross references - MT1G
ExoCarta ExoCarta_4495
Vesiclepedia VP_4495
Entrez Gene 4495
HGNC 7399
MIM 156353
UniProt P13640  
 MT1G identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MT1G
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    metal ion binding GO:0046872 IBA
Biological Process
    intracellular zinc ion homeostasis GO:0006882 IBA
    detoxification of copper ion GO:0010273 IBA
    monocyte differentiation GO:0030224 NAS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEP
    monocyte activation GO:0042117 NAS
    negative regulation of growth GO:0045926 IDA
    cellular response to cadmium ion GO:0071276 IBA
    cellular response to cadmium ion GO:0071276 IEP
    cellular response to copper ion GO:0071280 IBA
    cellular response to copper ion GO:0071280 IEP
    cellular response to zinc ion GO:0071294 IBA
    cellular response to zinc ion GO:0071294 IDA
    cellular response to zinc ion GO:0071294 IEP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified MT1G in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MT1G
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SHBG 6462
Two-hybrid Homo sapiens
2 SPINK7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 MT1M 4499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MT1G is involved
PathwayEvidenceSource
Cellular responses to stimuli IEA Reactome
Metallothioneins bind metals IEA Reactome
Response to metal ions IEA Reactome





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