Gene description for NRF1
Gene name nuclear respiratory factor 1
Gene symbol NRF1
Other names/aliases ALPHA-PAL
Species Homo sapiens
 Database cross references - NRF1
ExoCarta ExoCarta_4899
Vesiclepedia VP_4899
Entrez Gene 4899
HGNC 7996
MIM 600879
UniProt Q16656  
 NRF1 identified in sEVs derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for NRF1
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 ISS
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IMP
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 ISS
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified NRF1 in sEVs
1
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NRF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 KRT83  
Two-hybrid Homo sapiens
3 SLC22A18AS  
Two-hybrid Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 KPNA5 3841
Two-hybrid Homo sapiens
6 PRKCSH 5589
Co-fractionation Homo sapiens
7 ZBTB2 57621
Affinity Capture-MS Homo sapiens
8 USP7 7874
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CSNK2B 1460
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 PARP1 142
Reconstituted Complex Homo sapiens
11 FOXP3  
Affinity Capture-MS Homo sapiens
12 CKMT2  
Two-hybrid Homo sapiens
13 GLTSCR1L  
Two-hybrid Homo sapiens
14 SIAH2 6478
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
15 NFE2L1  
Affinity Capture-Western Homo sapiens
16 ASF1A 25842
Co-localization Homo sapiens
17 FOXJ2  
Affinity Capture-MS Homo sapiens
18 POGZ 23126
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 C3orf36  
Two-hybrid Homo sapiens
20 VEZF1  
Two-hybrid Homo sapiens
21 BBS2 583
Two-hybrid Homo sapiens
22 CSNK2A1 1457
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
23 TGM2 7052
Co-fractionation Homo sapiens
24 SPSB2  
Two-hybrid Homo sapiens
25 KIAA0907  
Two-hybrid Homo sapiens
26 MKI67  
Affinity Capture-MS Homo sapiens
27 PPRC1  
Reconstituted Complex Homo sapiens
28 BRWD1  
Two-hybrid Homo sapiens
29 SP4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 COL10A1  
Two-hybrid Homo sapiens
31 ESM1 11082
Two-hybrid Homo sapiens
32 L3MBTL3  
Two-hybrid Homo sapiens
33 ZMYM6  
Two-hybrid Homo sapiens
34 MAFF 23764
Reconstituted Complex Homo sapiens
35 Dynll1 56455
Two-hybrid Mus musculus
36 AGR2 10551
Two-hybrid Homo sapiens
37 C10orf55  
Two-hybrid Homo sapiens
38 FOXK2  
Affinity Capture-MS Homo sapiens
39 EGFR 1956
Negative Genetic Homo sapiens
40 TRIM35 23087
Two-hybrid Homo sapiens
41 LHX3  
Two-hybrid Homo sapiens
42 KLF15  
Two-hybrid Homo sapiens
43 WDR59 79726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TRAF2 7186
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 CEBPB  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 HIRA  
Co-localization Homo sapiens
47 HHIPL1 84439
Two-hybrid Homo sapiens
48 UBN1  
Co-localization Homo sapiens
49 Dynlt1b  
Two-hybrid Mus musculus
50 DYNLL2 140735
Affinity Capture-MS Homo sapiens
51 MTL5  
Two-hybrid Homo sapiens
52 MYH10 4628
Co-fractionation Homo sapiens
53 PPARGC1A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 FHL2 2274
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
55 C1orf64  
Two-hybrid Homo sapiens
56 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 LHX6  
Two-hybrid Homo sapiens
58 TEX43  
Two-hybrid Homo sapiens
59 SP2  
Two-hybrid Homo sapiens
60 PLXDC1  
Two-hybrid Homo sapiens
61 LMO4  
Two-hybrid Homo sapiens
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