Gene description for Capza2
Gene name capping protein (actin filament) muscle Z-line, alpha 2
Gene symbol Capza2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Capza2
ExoCarta ExoCarta_493810
Vesiclepedia VP_493810
Entrez Gene 493810
UniProt Q3T1K5  
 Capza2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Capza2
Molecular Function
    actin binding GO:0003779 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IEA
Subcellular Localization
    brush border GO:0005903 ISO
    F-actin capping protein complex GO:0008290 IBA
    F-actin capping protein complex GO:0008290 IEA
    F-actin capping protein complex GO:0008290 ISO
    membrane GO:0016020 ISO
    cortical cytoskeleton GO:0030863 IBA
    cortical cytoskeleton GO:0030863 ISO
 Experiment description of studies that identified Capza2 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Capza2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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