Gene description for Fasn
Gene name fatty acid synthase
Gene symbol Fasn
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Fasn
ExoCarta ExoCarta_50671
Vesiclepedia VP_50671
Entrez Gene 50671
UniProt P12785  
 Fasn identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Fasn
Molecular Function
    fatty acid synthase activity GO:0004312 IBA
    fatty acid synthase activity GO:0004312 IDA
    fatty acid synthase activity GO:0004312 IEA
    fatty acid synthase activity GO:0004312 ISO
    [acyl-carrier-protein] S-acetyltransferase activity GO:0004313 IEA
    [acyl-carrier-protein] S-acetyltransferase activity GO:0004313 ISO
    [acyl-carrier-protein] S-malonyltransferase activity GO:0004314 IMP
    3-oxoacyl-[acyl-carrier-protein] synthase activity GO:0004315 IEA
    3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity GO:0004316 IEA
    protein binding GO:0005515 IPI
    fatty acyl-[ACP] hydrolase activity GO:0016297 IEA
    oxidoreductase activity GO:0016491 IEA
    hydrolase activity GO:0016787 IEA
    lyase activity GO:0016829 IEA
    (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity GO:0019171 IEA
    phosphopantetheine binding GO:0031177 IEA
    small molecule binding GO:0036094 ISO
    identical protein binding GO:0042802 IPI
    enoyl-[acyl-carrier-protein] reductase (NADPH) activity GO:0141148 IEA
Biological Process
    glandular epithelial cell development GO:0002068 IEA
    glandular epithelial cell development GO:0002068 ISO
    acetyl-CoA metabolic process GO:0006084 IDA
    fatty acid biosynthetic process GO:0006633 IBA
    fatty acid biosynthetic process GO:0006633 IDA
    fatty acid biosynthetic process GO:0006633 IEA
    fatty acid biosynthetic process GO:0006633 ISO
    inflammatory response GO:0006954 IEA
    inflammatory response GO:0006954 ISO
    response to nutrient GO:0007584 IEP
    lipid biosynthetic process GO:0008610 ISO
    lipid biosynthetic process GO:0008610 TAS
    ether lipid biosynthetic process GO:0008611 IEA
    ether lipid biosynthetic process GO:0008611 ISO
    tissue development GO:0009888 ISO
    neutrophil differentiation GO:0030223 IEA
    neutrophil differentiation GO:0030223 ISO
    monocyte differentiation GO:0030224 IEA
    monocyte differentiation GO:0030224 ISO
    mammary gland development GO:0030879 IEA
    mammary gland development GO:0030879 ISO
    response to nutrient levels GO:0031667 IEP
    modulation by host of viral process GO:0044788 IEA
    cellular response to interleukin-4 GO:0071353 IEA
    cellular response to interleukin-4 GO:0071353 ISO
    establishment of endothelial intestinal barrier GO:0090557 IEA
    establishment of endothelial intestinal barrier GO:0090557 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    melanosome GO:0042470 IEA
    glycogen granule GO:0042587 IEA
    glycogen granule GO:0042587 ISO
 Experiment description of studies that identified Fasn in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Fasn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Rad23b  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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