Gene description for ABHD5
Gene name abhydrolase domain containing 5
Gene symbol ABHD5
Other names/aliases CDS
CGI58
IECN2
NCIE2
Species Homo sapiens
 Database cross references - ABHD5
ExoCarta ExoCarta_51099
Vesiclepedia VP_51099
Entrez Gene 51099
HGNC 21396
MIM 604780
UniProt Q8WTS1  
 ABHD5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ABHD5
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IDA
    triacylglycerol lipase activity GO:0004806 ISS
    protein binding GO:0005515 IPI
    lysophosphatidic acid acyltransferase activity GO:0042171 IBA
    lysophosphatidic acid acyltransferase activity GO:0042171 IDA
    carboxylic ester hydrolase activity GO:0052689 IBA
    lipase activator activity GO:0060229 IEA
Biological Process
    fatty acid metabolic process GO:0006631 IEA
    phosphatidic acid biosynthetic process GO:0006654 IBA
    phosphatidic acid biosynthetic process GO:0006654 IDA
    negative regulation of sequestering of triglyceride GO:0010891 IBA
    negative regulation of sequestering of triglyceride GO:0010891 IDA
    positive regulation of triglyceride catabolic process GO:0010898 IBA
    positive regulation of triglyceride catabolic process GO:0010898 IDA
    cell differentiation GO:0030154 IEA
    lipid homeostasis GO:0055088 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 IBA
    lipid droplet GO:0005811 IBA
    lipid droplet GO:0005811 IDA
    lipid droplet GO:0005811 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ABHD5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ABHD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PNPLA2  
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
PCA Homo sapiens
2 TMEM38B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SKAP2 8935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NRAS 4893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KRAS 3845
Synthetic Lethality Homo sapiens
6 SNRK  
Affinity Capture-MS Homo sapiens
7 PLIN1  
PCA Homo sapiens
FRET Homo sapiens
PCA Homo sapiens
FRET Homo sapiens
8 CCR1  
Affinity Capture-MS Homo sapiens
9 ZPR1 8882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SARAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HMOX2 3163
Affinity Capture-MS Homo sapiens
12 KDM4A  
Affinity Capture-MS Homo sapiens
13 TRIM59  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ABHD5 is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Triglyceride catabolism IEA Reactome
Triglyceride metabolism IEA Reactome





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