Gene description for RAB9B
Gene name RAB9B, member RAS oncogene family
Gene symbol RAB9B
Other names/aliases RAB9L
Rab-9L
Species Homo sapiens
 Database cross references - RAB9B
ExoCarta ExoCarta_51209
Vesiclepedia VP_51209
Entrez Gene 51209
HGNC 14090
MIM 300285
UniProt Q9NP90  
 RAB9B identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RAB9B
Molecular Function
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 IDA
    identical protein binding GO:0042802 IEA
Biological Process
    protein transport GO:0015031 IEA
    Rab protein signal transduction GO:0032482 IEA
    retrograde transport, endosome to Golgi GO:0042147 IBA
Subcellular Localization
    lysosome GO:0005764 IBA
    late endosome GO:0005770 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    secretory granule membrane GO:0030667 TAS
    phagocytic vesicle membrane GO:0030670 IEA
    phagocytic vesicle GO:0045335 IBA
    phagocytic vesicle GO:0045335 IDA
 Experiment description of studies that identified RAB9B in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAB9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHML  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FAM26D  
Affinity Capture-MS Homo sapiens
3 RABGGTA 5875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ASPRV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 FBXL6  
Affinity Capture-MS Homo sapiens
6 KIF14 9928
Affinity Capture-MS Homo sapiens
7 PRPH2 5961
Affinity Capture-MS Homo sapiens
8 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 POF1B 79983
Affinity Capture-MS Homo sapiens
10 CDSN 1041
Affinity Capture-MS Homo sapiens
11 VSIG8  
Affinity Capture-MS Homo sapiens
12 S100A3  
Affinity Capture-MS Homo sapiens
13 SBSN 374897
Affinity Capture-MS Homo sapiens
14 SERPINB5 5268
Affinity Capture-MS Homo sapiens
15 HAL 3034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PHACTR4 65979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 C16orf58 64755
Affinity Capture-MS Homo sapiens
18 SELENBP1 8991
Affinity Capture-MS Homo sapiens
19 DSG4 147409
Affinity Capture-MS Homo sapiens
20 SLC12A6 9990
Affinity Capture-MS Homo sapiens
21 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CHM 1121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CPA4 51200
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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