Gene description for GDE1
Gene name glycerophosphodiester phosphodiesterase 1
Gene symbol GDE1
Other names/aliases 363E6.2
MIR16
Species Homo sapiens
 Database cross references - GDE1
ExoCarta ExoCarta_51573
Vesiclepedia VP_51573
Entrez Gene 51573
HGNC 29644
MIM 605943
UniProt Q9NZC3  
 GDE1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GDE1
Molecular Function
    lysophospholipase activity GO:0004622 ISS
    protein binding GO:0005515 IPI
    glycerophosphodiester phosphodiesterase activity GO:0008889 IBA
    glycerophosphodiester phosphodiesterase activity GO:0008889 ISS
    glycerophosphodiester phosphodiesterase activity GO:0008889 TAS
    metal ion binding GO:0046872 IEA
    glycerophosphoinositol glycerophosphodiesterase activity GO:0047395 ISS
    glycerophosphoinositol glycerophosphodiesterase activity GO:0047395 TAS
Biological Process
    ethanolamine metabolic process GO:0006580 IBA
    ethanolamine metabolic process GO:0006580 ISS
    lipid metabolic process GO:0006629 TAS
    phospholipid metabolic process GO:0006644 IBA
    phospholipid metabolic process GO:0006644 ISS
    glycerophospholipid catabolic process GO:0046475 TAS
    N-acylethanolamine metabolic process GO:0070291 IBA
    N-acylethanolamine metabolic process GO:0070291 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    cytoplasmic vesicle membrane GO:0030659 IEA
 Experiment description of studies that identified GDE1 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GDE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SLC4A7 9497
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RNF126  
Affinity Capture-MS Homo sapiens
4 CYB5B 80777
Affinity Capture-MS Homo sapiens
5 CLGN 1047
Two-hybrid Homo sapiens
6 ADRA1A  
Two-hybrid Homo sapiens
7 ATL3 25923
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GAPDHS 26330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RGS2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 PLSCR1 5359
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ATAD3C 219293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
13 GAP43  
Affinity Capture-MS Homo sapiens
14 FAM84B 157638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DAZAP2  
Affinity Capture-MS Homo sapiens
16 RGS16 6004
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 UBAC2 337867
Affinity Capture-MS Homo sapiens
18 RPA3 6119
Proximity Label-MS Homo sapiens
19 CDK14 5218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 APOA2 336
Affinity Capture-MS Homo sapiens
21 ADCY2 108
Affinity Capture-MS Homo sapiens
22 TMEM109 79073
Affinity Capture-MS Homo sapiens
23 CXorf58  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PROCR 10544
Affinity Capture-MS Homo sapiens
25 BRI3BP 140707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 REEP6  
Affinity Capture-MS Homo sapiens
27 XPNPEP1 7511
Affinity Capture-MS Homo sapiens
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