Gene description for ERCC6L
Gene name excision repair cross-complementation group 6-like
Gene symbol ERCC6L
Other names/aliases PICH
RAD26L
Species Homo sapiens
 Database cross references - ERCC6L
ExoCarta ExoCarta_54821
Vesiclepedia VP_54821
Entrez Gene 54821
HGNC 20794
MIM 300687
UniProt Q2NKX8  
 ERCC6L identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for ERCC6L
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    DNA translocase activity GO:0015616 IBA
    DNA translocase activity GO:0015616 IDA
    ATP hydrolysis activity GO:0016887 IEA
    double-stranded DNA helicase activity GO:0036121 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    DNA repair GO:0006281 IBA
    chromatin remodeling GO:0006338 IEA
    DNA duplex unwinding GO:0032508 IEA
    cell division GO:0051301 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    kinetochore GO:0000776 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ERCC6L in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ERCC6L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBXN6 80700
Affinity Capture-MS Homo sapiens
2 CDK1 983
Biochemical Activity Homo sapiens
3 EIF4B 1975
Affinity Capture-MS Homo sapiens
4 NAGK 55577
Affinity Capture-MS Homo sapiens
5 MINK1 50488
Affinity Capture-MS Homo sapiens
6 SNX27 81609
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 FBXW11  
Affinity Capture-MS Homo sapiens
9 HSPA2 3306
Affinity Capture-MS Homo sapiens
10 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Ercc6l  
Affinity Capture-MS Mus musculus
12 MIDN 90007
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LMO4  
Two-hybrid Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 LDHA 3939
Affinity Capture-MS Homo sapiens
17 C14orf119  
Affinity Capture-MS Homo sapiens
18 FAS 355
Proximity Label-MS Homo sapiens
19 ZDHHC17 23390
Two-hybrid Homo sapiens
20 FCHO1  
Affinity Capture-MS Homo sapiens
21 CCT8L2  
Affinity Capture-MS Homo sapiens
22 NR3C1 2908
Proximity Label-MS Homo sapiens
23 SQSTM1 8878
Proximity Label-MS Homo sapiens
24 BRD2  
Affinity Capture-MS Homo sapiens
25 RB1CC1 9821
Affinity Capture-MS Homo sapiens
26 FEM1A  
Affinity Capture-MS Homo sapiens
27 METTL21B  
Affinity Capture-MS Homo sapiens
28 HSPA1A 3303
Affinity Capture-MS Homo sapiens
29 DUSP16  
Affinity Capture-MS Homo sapiens
30 AARSD1 80755
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
32 RPA4  
Proximity Label-MS Homo sapiens
33 HTT 3064
Two-hybrid Homo sapiens
34 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ACACA 31
Co-fractionation Homo sapiens
36 RDX 5962
Affinity Capture-MS Homo sapiens
37 RPA3 6119
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 TIMM8A 1678
Affinity Capture-MS Homo sapiens
39 BARD1 580
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 GOLGA1  
Proximity Label-MS Homo sapiens
42 SIX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 CHEK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
45 AMOT 154796
Proximity Label-MS Homo sapiens
46 PRKY  
Affinity Capture-MS Homo sapiens
47 SPDYE4  
Affinity Capture-MS Homo sapiens
48 CLPB 81570
Affinity Capture-MS Homo sapiens
49 CCDC96  
Affinity Capture-MS Homo sapiens
50 ALOX5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PASK  
Affinity Capture-MS Homo sapiens
52 Plk1  
Affinity Capture-MS Mus musculus
53 CDC5L 988
Affinity Capture-MS Homo sapiens
54 CAPN2 824
Affinity Capture-MS Homo sapiens
55 SIX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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