Gene description for WIPI1
Gene name WD repeat domain, phosphoinositide interacting 1
Gene symbol WIPI1
Other names/aliases ATG18
ATG18A
WIPI49
Species Homo sapiens
 Database cross references - WIPI1
ExoCarta ExoCarta_55062
Vesiclepedia VP_55062
Entrez Gene 55062
HGNC 25471
MIM 609224
UniProt Q5MNZ9  
 WIPI1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WIPI1
Molecular Function
    signaling receptor binding GO:0005102 IDA
    protein binding GO:0005515 IPI
    nuclear estrogen receptor binding GO:0030331 IDA
    protein-macromolecule adaptor activity GO:0030674 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    nuclear androgen receptor binding GO:0050681 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    autophagosome assembly GO:0000045 IMP
    autophagy of mitochondrion GO:0000422 IBA
    autophagy GO:0006914 IEP
    cellular response to starvation GO:0009267 IDA
    autophagy of peroxisome GO:0030242 IBA
    protein localization to phagophore assembly site GO:0034497 IBA
    nucleophagy GO:0044804 IBA
    vesicle targeting, trans-Golgi to endosome GO:0048203 IDA
    glycophagy GO:0061723 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IBA
    positive regulation of autophagosome assembly GO:2000786 IDA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    phagophore assembly site GO:0000407 IDA
    autophagosome membrane GO:0000421 IDA
    cytoplasm GO:0005737 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    endosome membrane GO:0010008 IDA
    clathrin-coated vesicle GO:0030136 IEA
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 IDA
 Experiment description of studies that identified WIPI1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WIPI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPL41 64975
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 AK4 205
Affinity Capture-MS Homo sapiens
4 MRPS7 51081
Affinity Capture-MS Homo sapiens
5 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
6 ATG2B 55102
Affinity Capture-MS Homo sapiens
7 COG1 9382
Affinity Capture-MS Homo sapiens
8 AMBRA1  
Affinity Capture-Western Homo sapiens
9 ATG9A 79065
Affinity Capture-Western Homo sapiens
10 MRPL35  
Affinity Capture-MS Homo sapiens
11 C16orf70  
Affinity Capture-MS Homo sapiens
12 DNASE2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MRPL13  
Affinity Capture-MS Homo sapiens
14 VPS35 55737
Co-localization Homo sapiens
15 ATG2A  
Affinity Capture-MS Homo sapiens
16 ACAA2 10449
Affinity Capture-MS Homo sapiens
17 S100A7 6278
Affinity Capture-MS Homo sapiens
18 MRPL37 51253
Affinity Capture-MS Homo sapiens
19 ATG101  
Affinity Capture-MS Homo sapiens
20 MRPL3  
Affinity Capture-MS Homo sapiens
21 FAM73B  
Affinity Capture-MS Homo sapiens
22 HGH1 51236
Affinity Capture-MS Homo sapiens
23 EDC3 80153
Affinity Capture-MS Homo sapiens
24 AMFR 267
Affinity Capture-MS Homo sapiens
25 ATG14  
Affinity Capture-MS Homo sapiens
26 LGALS7 3963
Affinity Capture-MS Homo sapiens
27 PKP1 5317
Affinity Capture-MS Homo sapiens
28 OPTN 10133
Affinity Capture-MS Homo sapiens
29 SSBP1 6742
Affinity Capture-MS Homo sapiens
30 IDH2 3418
Affinity Capture-MS Homo sapiens
31 ESR1  
Reconstituted Complex Homo sapiens
32 CECR5 27440
Affinity Capture-MS Homo sapiens
33 UBB 7314
Affinity Capture-MS Homo sapiens
34 BCAS3 54828
Affinity Capture-MS Homo sapiens
35 RPS20 6224
Proximity Label-MS Homo sapiens
36 HNRNPA3P1  
Affinity Capture-MS Homo sapiens
37 ABHD11 83451
Affinity Capture-MS Homo sapiens
38 ZNF185 7739
Affinity Capture-MS Homo sapiens
39 OSBPL5 114879
Affinity Capture-MS Homo sapiens
40 SCO1  
Affinity Capture-MS Homo sapiens
41 UQCRC1 7384
Affinity Capture-MS Homo sapiens
42 GOSR1 9527
Affinity Capture-MS Homo sapiens
43 UHRF1 29128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 GNB2L1 10399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NDUFA10 4705
Affinity Capture-MS Homo sapiens
46 CEP76  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 KCTD15 79047
Two-hybrid Homo sapiens
48 ZNF787  
Affinity Capture-MS Homo sapiens
49 SCO2  
Affinity Capture-MS Homo sapiens
50 AR 367
Reconstituted Complex Homo sapiens
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