Gene description for TMEM100
Gene name transmembrane protein 100
Gene symbol TMEM100
Other names/aliases -
Species Homo sapiens
 Database cross references - TMEM100
ExoCarta ExoCarta_55273
Vesiclepedia VP_55273
Entrez Gene 55273
HGNC 25607
MIM 616334
UniProt Q9NV29  
 TMEM100 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TMEM100
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    angiogenesis GO:0001525 IEA
    vasculogenesis GO:0001570 IEA
    in utero embryonic development GO:0001701 IEA
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 IEA
    Notch signaling pathway GO:0007219 IEA
    BMP signaling pathway GO:0030509 IBA
    BMP signaling pathway GO:0030509 IDA
    positive regulation of endothelial cell differentiation GO:0045603 ISS
    regulation of calcium-mediated signaling GO:0050848 IEA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IEA
    regulation of sensory perception of pain GO:0051930 IBA
    regulation of sensory perception of pain GO:0051930 ISS
    arterial endothelial cell differentiation GO:0060842 IEA
    cellular response to BMP stimulus GO:0071773 IDA
    positive regulation of vasculogenesis GO:2001214 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    perikaryon GO:0043204 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified TMEM100 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TMEM100
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLDN7 1366
Two-hybrid Homo sapiens
2 TMEM52B 120939
Two-hybrid Homo sapiens
3 F11R 50848
Two-hybrid Homo sapiens
4 GJA8  
Two-hybrid Homo sapiens
5 FNDC9  
Two-hybrid Homo sapiens
6 TMEM80  
Two-hybrid Homo sapiens
7 RNF19B  
Two-hybrid Homo sapiens
8 CCDC57 284001
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 SLC35A5  
Two-hybrid Homo sapiens
10 ERGIC3 51614
Two-hybrid Homo sapiens
11 TMEM173  
Two-hybrid Homo sapiens
12 CD33 945
Two-hybrid Homo sapiens
13 EBP  
Two-hybrid Homo sapiens
14 HSD17B13  
Two-hybrid Homo sapiens
15 JAGN1 84522
Two-hybrid Homo sapiens
16 SLC30A4 7782
Two-hybrid Homo sapiens
17 GPR152  
Two-hybrid Homo sapiens
18 FAM209A 200232
Two-hybrid Homo sapiens
19 FAM134C 162427
Two-hybrid Homo sapiens
20 STX1A 6804
Two-hybrid Homo sapiens
21 TMEM79  
Two-hybrid Homo sapiens
22 TMEM130  
Two-hybrid Homo sapiens
23 MEOX2  
Two-hybrid Homo sapiens
24 CREB3  
Two-hybrid Homo sapiens
25 C10orf54 64115
Two-hybrid Homo sapiens
26 NUP93 9688
Two-hybrid Homo sapiens
27 LHFPL5  
Two-hybrid Homo sapiens
28 RMDN3 55177
Two-hybrid Homo sapiens
29 TMEM167B  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which TMEM100 is involved
No pathways found





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