Gene description for RFK
Gene name riboflavin kinase
Gene symbol RFK
Other names/aliases RIFK
Species Homo sapiens
 Database cross references - RFK
ExoCarta ExoCarta_55312
Vesiclepedia VP_55312
Entrez Gene 55312
HGNC 30324
MIM 613010
UniProt Q969G6  
 RFK identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RFK
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    riboflavin kinase activity GO:0008531 IBA
    riboflavin kinase activity GO:0008531 IDA
    riboflavin kinase activity GO:0008531 NAS
    riboflavin kinase activity GO:0008531 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    riboflavin metabolic process GO:0006771 IBA
    riboflavin metabolic process GO:0006771 IDA
    riboflavin metabolic process GO:0006771 TAS
    apoptotic process GO:0006915 IMP
    riboflavin biosynthetic process GO:0009231 NAS
    FMN biosynthetic process GO:0009398 IBA
    FMN biosynthetic process GO:0009398 IEA
    positive regulation of NAD(P)H oxidase activity GO:0033864 IMP
    flavin adenine dinucleotide biosynthetic process GO:0072388 IEA
    reactive oxygen species metabolic process GO:0072593 IEA
Subcellular Localization
    cytoplasm GO:0005737 NAS
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified RFK in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RFK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLPP 8192
Proximity Label-MS Homo sapiens
2 GRHPR 9380
Co-fractionation Homo sapiens
3 KRAS 3845
Negative Genetic Homo sapiens
4 GOT2 2806
Co-fractionation Homo sapiens
5 GPI 2821
Co-fractionation Homo sapiens
6 SEC14L2  
Co-fractionation Homo sapiens
7 YKT6 10652
Co-fractionation Homo sapiens
8 MEMO1 51072
Co-fractionation Homo sapiens
9 EGFR 1956
Negative Genetic Homo sapiens
10 RAB1A 5861
Two-hybrid Homo sapiens
11 CREBBP  
Two-hybrid Homo sapiens
12 ALDH7A1 501
Co-fractionation Homo sapiens
13 NUDT19 390916
Co-fractionation Homo sapiens
14 PEBP1 5037
Co-fractionation Homo sapiens
15 IL1RN 3557
Affinity Capture-MS Homo sapiens
16 GOT1 2805
Co-fractionation Homo sapiens
17 UFM1 51569
Co-fractionation Homo sapiens
18 DNAJB9 4189
Two-hybrid Homo sapiens
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