Gene description for AP5M1
Gene name adaptor-related protein complex 5, mu 1 subunit
Gene symbol AP5M1
Other names/aliases C14orf108
MUDENG
Mu5
MuD
Species Homo sapiens
 Database cross references - AP5M1
ExoCarta ExoCarta_55745
Vesiclepedia VP_55745
Entrez Gene 55745
HGNC 20192
MIM 614368
UniProt Q9H0R1  
 AP5M1 identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for AP5M1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 NAS
    endosomal transport GO:0016197 IBA
    endosomal transport GO:0016197 IMP
Subcellular Localization
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 IEA
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IDA
    late endosome GO:0005770 NAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
    AP-type membrane coat adaptor complex GO:0030119 IBA
    AP-type membrane coat adaptor complex GO:0030119 IDA
    late endosome membrane GO:0031902 IEA
    AP-5 adaptor complex GO:0044599 NAS
 Experiment description of studies that identified AP5M1 in sEVs
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for AP5M1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ASB9  
Affinity Capture-MS Homo sapiens
2 HSPA12B 116835
Affinity Capture-MS Homo sapiens
3 AP5S1  
Affinity Capture-MS Homo sapiens
4 SPG11 80208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PAX6  
Affinity Capture-MS Homo sapiens
6 GTF3C3 9330
Affinity Capture-MS Homo sapiens
7 HSPA12A 259217
Affinity Capture-MS Homo sapiens
8 CKMT2  
Affinity Capture-MS Homo sapiens
9 AP5B1 91056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 DCAF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 Cep55 74107
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which AP5M1 is involved
No pathways found





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