Gene description for ASH1L
Gene name ash1 (absent, small, or homeotic)-like (Drosophila)
Gene symbol ASH1L
Other names/aliases ASH1
ASH1L1
KMT2H
Species Homo sapiens
 Database cross references - ASH1L
ExoCarta ExoCarta_55870
Vesiclepedia VP_55870
Entrez Gene 55870
HGNC 19088
MIM 607999
UniProt Q9NR48  
 ASH1L identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ASH1L
Molecular Function
    DNA binding GO:0003677 IEA
    chromatin binding GO:0003682 IEA
    histone H3K4 methyltransferase activity GO:0042800 IBA
    metal ion binding GO:0046872 IEA
    histone H3K9 methyltransferase activity GO:0046974 ISS
    histone H3K36 methyltransferase activity GO:0046975 IDA
    histone H3 methyltransferase activity GO:0140938 TAS
    histone H3K9me2 methyltransferase activity GO:0140947 IEA
    histone H3K9 monomethyltransferase activity GO:0140948 IEA
    histone H3K36 trimethyltransferase activity GO:0140955 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    skeletal system development GO:0001501 IEA
    negative regulation of acute inflammatory response GO:0002674 IEA
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IBA
    transcription by RNA polymerase II GO:0006366 IEA
    inflammatory response GO:0006954 IEA
    single fertilization GO:0007338 IEA
    post-embryonic development GO:0009791 IEA
    flagellated sperm motility GO:0030317 IEA
    methylation GO:0032259 IEA
    negative regulation of MAPK cascade GO:0043409 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    decidualization GO:0046697 IEA
    uterus morphogenesis GO:0061038 IEA
    tarsal gland development GO:1903699 IEA
    uterine gland development GO:1903709 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    chromosome GO:0005694 IEA
    Golgi apparatus GO:0005794 IDA
    bicellular tight junction GO:0005923 IEA
 Experiment description of studies that identified ASH1L in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASH1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 FGFBP1 9982
Affinity Capture-MS Homo sapiens
3 OASL 8638
Affinity Capture-MS Homo sapiens
4 HIST1H3A 8350
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
5 SMAD7  
Two-hybrid Homo sapiens
6 RPS3A 6189
Cross-Linking-MS (XL-MS) Homo sapiens
7 H3F3A 3020
Affinity Capture-MS Homo sapiens
8 NXF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SIKE1  
Affinity Capture-MS Homo sapiens
10 MAGEB2 4113
Affinity Capture-MS Homo sapiens
11 NOP2 4839
Affinity Capture-MS Homo sapiens
12 RPS19 6223
Cross-Linking-MS (XL-MS) Homo sapiens
13 RPL15 6138
Affinity Capture-MS Homo sapiens
14 MCC 4163
Affinity Capture-MS Homo sapiens
15 MORF4L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 EFNA3  
Affinity Capture-MS Homo sapiens
17 PNMAL1  
Affinity Capture-MS Homo sapiens
18 NUP43 348995
Proximity Label-MS Homo sapiens
19 MORF4L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 EIF2B5 8893
Affinity Capture-MS Homo sapiens
21 SART1 9092
Cross-Linking-MS (XL-MS) Homo sapiens
22 THAP7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 OXTR 5021
Two-hybrid Homo sapiens
24 GID8 54994
Affinity Capture-MS Homo sapiens
25 RAN 5901
Cross-Linking-MS (XL-MS) Homo sapiens
26 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
27 RPL28 6158
Affinity Capture-MS Homo sapiens
28 CLEC11A 6320
Affinity Capture-MS Homo sapiens
29 RPL23A 6147
Affinity Capture-MS Homo sapiens
30 Ufl1  
Affinity Capture-MS Mus musculus
31 MAGEA9  
Affinity Capture-MS Homo sapiens
32 BRPF3  
Affinity Capture-MS Homo sapiens
33 PARD6A  
Affinity Capture-MS Homo sapiens
34 Set 56086
Affinity Capture-MS Mus musculus
35 CDX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LURAP1  
Affinity Capture-MS Homo sapiens
37 KDM5C  
Affinity Capture-MS Homo sapiens
38 RBBP4 5928
Affinity Capture-MS Homo sapiens
39 PIPSL 266971
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ASH1L is involved
PathwayEvidenceSource
Chromatin modifying enzymes TAS Reactome
Chromatin organization TAS Reactome
PKMTs methylate histone lysines TAS Reactome





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