Gene description for Nptn
Gene name neuroplastin
Gene symbol Nptn
Other names/aliases Sdfr1
Species Rattus norvegicus
 Database cross references - Nptn
ExoCarta ExoCarta_56064
Vesiclepedia VP_56064
Entrez Gene 56064
UniProt P97546  
 Nptn identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Nptn
Molecular Function
    type 1 fibroblast growth factor receptor binding GO:0005105 IDA
    transmembrane transporter binding GO:0044325 IPI
    cell adhesion molecule binding GO:0050839 IDA
    cell-cell adhesion mediator activity GO:0098632 IBA
Biological Process
    negative regulation of cytokine production GO:0001818 IEA
    negative regulation of cytokine production GO:0001818 ISO
    negative regulation of cytokine production GO:0001818 ISS
    positive regulation of protein phosphorylation GO:0001934 IDA
    intracellular calcium ion homeostasis GO:0006874 IEA
    intracellular calcium ion homeostasis GO:0006874 ISO
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IBA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IDA
    axon guidance GO:0007411 IBA
    visual learning GO:0008542 IDA
    positive regulation of neuron projection development GO:0010976 IDA
    regulation of cell adhesion GO:0030155 TAS
    positive regulation of fibroblast growth factor receptor signaling pathway GO:0045743 IDA
    positive regulation of long-term neuronal synaptic plasticity GO:0048170 IMP
    modulation of chemical synaptic transmission GO:0050804 IEA
    modulation of chemical synaptic transmission GO:0050804 ISO
    synapse organization GO:0050808 ISO
    long-term synaptic potentiation GO:0060291 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    dendrite self-avoidance GO:0070593 IBA
    trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission GO:0099557 EXP
    trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission GO:0099557 IDA
    positive regulation of long-term synaptic potentiation GO:1900273 IMP
    regulation of receptor localization to synapse GO:1902683 IEA
    regulation of receptor localization to synapse GO:1902683 ISO
    positive regulation of protein localization GO:1903829 IMP
    excitatory synapse assembly GO:1904861 IEA
    excitatory synapse assembly GO:1904861 ISO
Subcellular Localization
    immunological synapse GO:0001772 IEA
    immunological synapse GO:0001772 ISO
    immunological synapse GO:0001772 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IDA
    membrane GO:0016020 IEA
    membrane GO:0016020 TAS
    axon GO:0030424 IBA
    dendrite GO:0030425 IDA
    presynaptic membrane GO:0042734 IDA
    postsynaptic membrane GO:0045211 TAS
    presynaptic active zone membrane GO:0048787 EXP
    presynaptic active zone membrane GO:0048787 IDA
    inhibitory synapse GO:0060077 IDA
    synaptic membrane GO:0097060 IDA
    synaptic membrane GO:0097060 IDA
    Schaffer collateral - CA1 synapse GO:0098685 EXP
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    postsynaptic density membrane GO:0098839 IDA
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    GABA-ergic synapse GO:0098982 EXP
    GABA-ergic synapse GO:0098982 IDA
 Experiment description of studies that identified Nptn in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nptn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Nptn is involved
No pathways found





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